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PMID: 1139415 Published · ppublish English Journal Article

A simple method for rapid identification of Sphaerophorus necrophorus isolates.

Canadian journal of comparative medicine : Revue canadienne de medecine comparee ·Vol. 39 ·No. 3 ·1975-07-00 ·Pages 349-53

Simon PC

Abstract

A hemagglutination inhibition test for the rapid identification of Sphaerophorus necrophorus is described. Erythrocytes from six species of animals were tested and human cells were found to be the best agglutination indicators. Antiserum prepared in rabbits was found to be specific for S. necrophorus hemagglutinins when tested against 20 isolates of S. necrophorus and 117 other bacteria belonging to 22 genera. The possibility of using a hemagglutination inhibition test for the detection of bovine necrobacillosis was explored.

MeSH Terms
Animals Antibodies, Bacterial/isolation & purification Antigens, Bacterial/analysis Cattle Chickens Fusobacterium/isolation & purification Fusobacterium Infections/diagnosis Fusobacterium necrophorum/isolation & purification Guinea Pigs Hemagglutination Inhibition Tests/methods Humans Immune Sera Rabbits Sheep
Chemicals
Antibodies, Bacterial Antigens, Bacterial Immune Sera
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Simon P C
References (4)
4 references, click to expand
  1. A STUDY OF BACILLUS NECROPHORUS OBTAINED FROM COWS.
    J Bacteriol. 1930 Nov;20(5):343-60 PMID: 16559461
  2. [Procedure for differentiating between Spherophorus necrophorus (Schmorl 1891) and Spherophorus funduliformis (Halle 1898)].
    Ann Inst Pasteur (Paris). 1954 Mar;86(3):384-6 PMID: 13158972
  3. Cultivation and maintenance of Sphaerophorus necrophorus. I. An anaerobic medium for aerobic incubation.
    Can J Comp Med. 1974 Jan;38(1):94-6 PMID: 4272964
  4. Isolation of Sphaerophorus necrophorus from bovine hepatic abscesses in British Columbia.
    Can J Comp Med. 1971 Apr;35(2):103-6 PMID: 4253458
Article Info
Journal
Canadian journal of comparative medicine : Revue canadienne de medecine comparee
Abbr.
Can J Comp Med
ISSN
0008-4050
Published
1975-07-00
Pages
349-53
Language
English
Region
Canada
NLM ID
0151747
PMCID
PMC1277469
Subset
IM
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