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PMID: 11400430 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Escherichia coli initiation factor IF3 binding to AUG and AUG-containing single strands and hairpin loops, and nonspecific binding to polymers.

Biochimica et biophysica acta ·Vol. 349 ·No. 1 ·1974-04-27 ·Pages 125-30

Wickstrom E

Abstract

Nitrocellulose filter binding and equilibrium dialysis detected the binding of Escherichia coli initiation factor IF3 to AUG, An UGUm single strands and hairpin loops, poly(A,U,G), poly(U), and f2 RNA. No binding was detected for GUA, A8 U, or the hairpin loop A5 GC5 U5. AUG-specific binding, per nucleotide, is strong; nonspecific binding, per nucleotide, is weak.

MeSH Terms

Binding, Competitive Collodion DNA, Single-Stranded/metabolism Dialysis Escherichia coli Oligonucleotides/chemical synthesis,metabolism Peptide Initiation Factors/metabolism Polymers Polyribonucleotides/metabolism Prokaryotic Initiation Factor-3 Protein Binding Temperature Thermodynamics

Chemicals

DNA, Single-Stranded Oligonucleotides Peptide Initiation Factors Polymers Polyribonucleotides Prokaryotic Initiation Factor-3 poly AUG Collodion

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Wickstrom E

Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colo. 80302, USA.

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0005-2787(74)90015-x

Published

1974-04-27

Pages

125-30

Language

English

Region

Netherlands

NLM ID

0217513

Subset

Grants

NIGMS NIH HHS · GM-10840 · United States

External Links

PubMed source DOI: 10.1016/0005-2787(74)90015-x

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Escherichia coli initiation factor IF3 binding to AUG and AUG-containing single strands and hairpin loops, and nonspecific binding to polymers.

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