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PMID: 11408587 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mitotic phosphorylation of Golgi reassembly stacking protein 55 by mitogen-activated protein kinase ERK2.

Molecular biology of the cell ·Vol. 12 ·No. 6 ·2001-06-00 ·Pages 1811-7

Jesch SA, Lewis TS, Ahn NG, Linstedt AD

Abstract

The role of the mitogen-activated protein kinase kinase (MKK)/extracellular-activated protein kinase (ERK) pathway in mitotic Golgi disassembly is controversial, in part because Golgi-localized targets have not been identified. We observed that Golgi reassembly stacking protein 55 (GRASP55) was phosphorylated in mitotic cells and extracts, generating a mitosis-specific phospho-epitope recognized by the MPM2 mAb. This phosphorylation was prevented by mutation of ERK consensus sites in GRASP55. GRASP55 mitotic phosphorylation was significantly reduced, both in vitro and in vivo, by treatment with U0126, a potent and specific inhibitor of MKK and thus ERK activation. Furthermore, ERK2 directly phosphorylated GRASP55 on the same residues that generated the MPM2 phospho-epitope. These results are the first demonstration of GRASP55 mitotic phosphorylation and indicate that the MKK/ERK pathway directly phosphorylates the Golgi during mitosis.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal/metabolism Butadienes/pharmacology Cloning, Molecular DNA, Complementary/metabolism Enzyme Inhibitors/pharmacology Epitopes Glutathione Transferase/metabolism Golgi Apparatus/metabolism Golgi Matrix Proteins HeLa Cells Humans Membrane Proteins/metabolism Mitogen-Activated Protein Kinase 1/metabolism Mitogen-Activated Protein Kinases/metabolism Mitosis/physiology Molecular Sequence Data Mutation Nitriles/pharmacology Phosphorylation
Chemicals
Antibodies, Monoclonal Butadienes DNA, Complementary Enzyme Inhibitors Epitopes GORASP2 protein, human Golgi Matrix Proteins Membrane Proteins Nitriles U 0126 Glutathione Transferase Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jesch S A
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
Lewis T S
Ahn N G
Linstedt A D
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.12.6.1811
Published
2001-06-00
Pages
1811-7
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC37343
Subset
IM
Grants
NIGMS NIH HHS · R01 GM056779 · United States
NIGMS NIH HHS · GM-56779-02 · United States
External Links
PubMed source DOI: 10.1091/mbc.12.6.1811 PMC Full Text View journal details
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