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PMID: 1141438 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Binding of the products of prothrombin activation to human platelets.

The Journal of clinical investigation ·Vol. 56 ·No. 1 ·1975-07-00 ·Pages 241-5

Tollefsen DM, Jackson CM, Majerus PW

Abstract

Binding of prothrombin and activation intermediates 1 and 2 to human platelets was tested with 125-I-labeled protein preparations. None of these precursors of thrombin bound to platelets under conditions in which high affinity binding of thrombin was observed,nor did they cause platelet aggregation or serotonin release. The molecular conformation required for binding to platelets as well as for induction of platelet aggregation and release is present, therefore, only after the final step in prothrombin activation.

MeSH Terms
Binding Sites Blood Platelets/drug effects,metabolism Calcium/pharmacology Carbon Radioisotopes Electrophoresis, Polyacrylamide Gel Humans In Vitro Techniques Iodine Radioisotopes Molecular Conformation Platelet Aggregation Protein Binding Prothrombin/metabolism Serotonin/metabolism Thrombin/metabolism
Chemicals
Carbon Radioisotopes Iodine Radioisotopes Serotonin Prothrombin Thrombin Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tollefsen D M
Jackson C M
Majerus P W
References (10)
10 references, click to expand
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    J Biol Chem. 1974 Apr 25;249(8):2646-51 PMID: 4856653
  7. The conversion of prothrombin to thrombin. II. Differentiation between thrombin- and factor Xa-catalyzed proteolyses.
    J Biol Chem. 1974 Jan 25;249(2):606-11 PMID: 4809531
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    J Biol Chem. 1974 Jan 25;249(2):594-605 PMID: 4809530
  9. Inhibition of human platelet aggregation by monovalent antifibrinogen antibody fragments.
    J Clin Invest. 1975 Jun;55(6):1259-68 PMID: 1169259
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI108075
Published
1975-07-00
Pages
241-5
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC436576
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI108075 PMC Full Text View journal details
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