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PMID: 11425879 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleocytoplasmic shuttling of endocytic proteins.

The Journal of cell biology ·Vol. 153 ·No. 7 ·2001-06-25 ·Pages 1511-7

Vecchi M, Polo S, Poupon V, van de Loo JW, Benmerah A, Di Fiore PP

Abstract

Many cellular processes rely on the ordered assembly of macromolecular structures. Here, we uncover an unexpected link between two such processes, endocytosis and transcription. Many endocytic proteins, including eps15, epsin1, the clathrin assembly lymphoid myeloid leukemia (CALM), and alpha-adaptin, accumulate in the nucleus when nuclear export is inhibited. Endocytosis and nucleocytoplasmic shuttling of endocytic proteins are apparently independent processes, since inhibition of endocytosis did not appreciably alter nuclear translocation of endocytic proteins, and blockade of nuclear export did not change the initial rate of endocytosis. In the nucleus, eps15 and CALM acted as positive modulators of transcription in a GAL4-based transactivation assay, thus raising the intriguing possibility that some endocytic proteins play a direct or indirect role in transcriptional regulation.

MeSH Terms
Active Transport, Cell Nucleus/physiology Adaptor Protein Complex alpha Subunits Adaptor Proteins, Signal Transducing Adaptor Proteins, Vesicular Transport Animals Calcium-Binding Proteins/metabolism Carrier Proteins/metabolism Cell Line Cell Nucleus/metabolism Chlorocebus aethiops Cytoplasm/metabolism DNA-Binding Proteins Endocytosis/physiology Fungal Proteins/genetics,metabolism HeLa Cells Humans Intracellular Signaling Peptides and Proteins Membrane Proteins/metabolism Monomeric Clathrin Assembly Proteins Nerve Tissue Proteins/metabolism Neuropeptides/metabolism Phosphoproteins/metabolism Protein Transport/physiology Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae Proteins Signal Transduction/physiology Transcription Factors/genetics,metabolism Transcription, Genetic/physiology Transcriptional Activation/physiology Vesicular Transport Proteins
Chemicals
Adaptor Protein Complex alpha Subunits Adaptor Proteins, Signal Transducing Adaptor Proteins, Vesicular Transport Calcium-Binding Proteins Carrier Proteins DNA-Binding Proteins EPS15 protein, human Fungal Proteins GAL4 protein, S cerevisiae Intracellular Signaling Peptides and Proteins Membrane Proteins Monomeric Clathrin Assembly Proteins Nerve Tissue Proteins Neuropeptides PICALM protein, human Phosphoproteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors Vesicular Transport Proteins epsin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vecchi M
Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy.
Polo S
Poupon V
van de Loo J W
Benmerah A
Di Fiore P P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.153.7.1511
Published
2001-06-25
Pages
1511-7
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2150719
Subset
IM
Grants
Telethon · D.090 · Italy
External Links
PubMed source DOI: 10.1083/jcb.153.7.1511 PMC Full Text View journal details
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