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PMID: 11445536 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The ATM-related domain of TRRAP is required for histone acetyltransferase recruitment and Myc-dependent oncogenesis.

Genes & development ·Vol. 15 ·No. 13 ·2001-07-01 ·Pages 1619-24

Park J, Kunjibettu S, McMahon SB, Cole MD

Abstract

The ATM-related TRRAP protein is a component of several different histone acetyltransferase (HAT) complexes but lacks the kinase activity characteristic of other ATM family members. We identified a novel function for this evolutionarily conserved domain in its requirement for the assembly of a functional HAT complex. Ectopic expression of TRRAP protein with a mutation in the ATM-related domain inhibits Myc-mediated oncogenic transformation. The Myc-binding region of TRRAP maps to a separable domain, and ectopic expression of this domain inhibits cell growth. These findings demonstrate that the ATM-related domain of TRRAP forms a structural core for the assembly and recruitment of HAT complexes by transcriptional activators.

MeSH Terms
Acetylation Acetyltransferases/metabolism Adaptor Proteins, Signal Transducing Animals Ataxia Telangiectasia Mutated Proteins Binding Sites Carrier Proteins/metabolism Cell Cycle Proteins Cell Line Cell Transformation, Neoplastic DNA-Binding Proteins Histone Acetyltransferases Histones/metabolism Humans Nuclear Proteins/genetics,metabolism Protein Serine-Threonine Kinases/metabolism Proto-Oncogene Proteins c-myc/genetics,metabolism Rats Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae Proteins Trans-Activators/metabolism Transcription Factors Tumor Suppressor Proteins p300-CBP Transcription Factors
Chemicals
Adaptor Proteins, Signal Transducing Carrier Proteins Cell Cycle Proteins DNA-Binding Proteins Histones Nuclear Proteins Proto-Oncogene Proteins c-myc Recombinant Fusion Proteins SUPT3H protein, human Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Tumor Suppressor Proteins transformation-transcription domain-associated protein Acetyltransferases Histone Acetyltransferases p300-CBP Transcription Factors p300-CBP-associated factor ATM protein, human Ataxia Telangiectasia Mutated Proteins Protein Serine-Threonine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Park J
Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, New Jersey 08544-1014, USA.
Kunjibettu S
McMahon S B
Cole M D
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.900101
Published
2001-07-01
Pages
1619-24
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312730
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.900101 PMC Full Text View journal details
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