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PMID: 11447110 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Importin 13: a novel mediator of nuclear import and export.

The EMBO journal ·Vol. 20 ·No. 14 ·2001-07-16 ·Pages 3685-94

Mingot JM, Kostka S, Kraft R, Hartmann E, Görlich D

Abstract

Importin beta-related receptors mediate translocation through nuclear pore complexes. Co-operation with the RanGTPase system allows them to bind and subsequently release their substrates on opposite sides of the nuclear envelope, which in turn ensures a directed nucleocytoplasmic transport. Here we identify a novel family member from higher eukaryotes that functions primarily, but not exclusively, in import. It accounts for nuclear accumulation of the SUMO-1/sentrin-conjugating enzyme hUBC9 and mediates import of the RBM8 (Y14) protein, and is therefore referred to as importin 13 (Imp13). Unexpectedly, Imp13 also shows export activity towards the translation initiation factor eIF1A and is thus a case where a single importin beta-like receptor transports different substrates in opposite directions. However, Imp13 operates differently from typical exportins in that the binding of eIF1A to Imp13 is only regulated indirectly by RanGTP, and the cytoplasmic release of eIF1A from Imp13 is triggered by the loading of import substrates onto Imp13.

MeSH Terms
Cell Nucleus/metabolism Eukaryotic Initiation Factor-1 HeLa Cells Humans Karyopherins Ligases/metabolism Molecular Sequence Data Nuclear Proteins/physiology Peptide Initiation Factors/metabolism Protein Transport/physiology RNA-Binding Proteins/metabolism SUMO-1 Protein Ubiquitin-Conjugating Enzymes Ubiquitins/metabolism
Chemicals
Eukaryotic Initiation Factor-1 Karyopherins Nuclear Proteins Peptide Initiation Factors RBM8A protein, human RNA-Binding Proteins SUMO-1 Protein Ubiquitins eukaryotic peptide initiation factor-1A Ubiquitin-Conjugating Enzymes Ligases ubiquitin-conjugating enzyme UBC9
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mingot J M
ZMBH, INF 282, D-69120 Heidelberg, Germany.
Kostka S
Kraft R
Hartmann E
Görlich D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.14.3685
Published
2001-07-16
Pages
3685-94
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125545
Subset
IM
Databases
GENBANK
AF267987
External Links
PubMed source DOI: 10.1093/emboj/20.14.3685 PMC Full Text View journal details
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