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PMID: 11449048 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis.

The Plant cell ·Vol. 13 ·No. 7 ·2001-07-00 ·Pages 1511-26

Jin JB, Kim YA, Kim SJ, Lee SH, Kim DH, Cheong GW, Hwang I

Abstract

Dynamin, a high-molecular-weight GTPase, plays a critical role in vesicle formation at the plasma membrane during endocytosis in animal cells. Here we report the identification of a new dynamin homolog in Arabidopsis named Arabidopsis dynamin-like 6 (ADL6). ADL6 is quite similar to dynamin I in its structural organization: a conserved GTPase domain at the N terminus, a pleckstrin homology domain at the center, and a Pro-rich motif at the C terminus. In the cell, a majority of ADL6 is associated with membranes. Immunohistochemistry and in vivo targeting experiments revealed that ADL6 is localized to the Golgi apparatus. Expression of the dominant negative mutant ADL6[K51E] in Arabidopsis protoplasts inhibited trafficking of cargo proteins destined for the lytic vacuole and caused them to accumulate at the trans-Golgi network. In contrast, expression of ADL6[K51E] did not affect trafficking of a cargo protein, H(+)-ATPase:green fluorescent protein, destined for the plasma membrane. These results suggest that ADL6 is involved in vesicle formation for vacuolar trafficking at the trans-Golgi network but not for trafficking to the plasma membrane in plant cells.

MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics,metabolism Arabidopsis Proteins Cell Membrane/metabolism Dynamin I Dynamins GTP Phosphohydrolases/chemistry,genetics,metabolism Immunohistochemistry Microfilament Proteins/metabolism Molecular Sequence Data Mutation Plant Proteins/chemistry,genetics,metabolism Protein Conformation Protein Transport Proton-Translocating ATPases/metabolism Recombinant Fusion Proteins/analysis Sequence Analysis Vacuoles/metabolism,ultrastructure trans-Golgi Network/enzymology,ultrastructure
Chemicals
Arabidopsis Proteins Microfilament Proteins Plant Proteins Recombinant Fusion Proteins Dynamin I ADL6 protein, Arabidopsis GTP Phosphohydrolases Proton-Translocating ATPases Dynamins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Jin J B
Department of Molecular Biology, Gyeongsang National University, Chinju 660-701, Korea.
Kim Y A
Kim S J
Lee S H
Kim D H
Cheong G W
Hwang I
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.000534
Published
2001-07-00
Pages
1511-26
Language
English
Region
England
NLM ID
9208688
PMCID
PMC139540
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.000534 PMC Full Text View journal details
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