Home LiteratureArticle Details
PMID: 11463849 Published · ppublish English Journal Article

A murine homologue of the Drosophila brainiac gene shows homology to glycosyltransferases and is required for preimplantation development of the mouse.

Molecular and cellular biology ·Vol. 21 ·No. 16 ·2001-08-00 ·Pages 5688-97

Vollrath B, Fitzgerald KJ, Leder P

Abstract

The neurogenic gene brainiac was first isolated in Drosophila melanogaster, where it interacts genetically with members of the Notch signaling cascade. We have isolated a murine homologue of the Drosophila brainiac gene and delineated its highly specific expression pattern during development and adult life. We find particularly strong expression in the developing central nervous system, in the developing retina, and in the adult hippocampus. Targeted deletion of mouse Brainiac 1 expression leads to embryonic lethality prior to implantation. Null embryos can be recovered as blastocysts but do not appear to implant, indicating that mouse Brainiac 1, likely a glycosyltransferase, is crucial for very early development of the mouse embryo.

MeSH Terms
Amino Acid Sequence Animals Drosophila Embryonic and Fetal Development/genetics Gene Expression Regulation, Developmental Genes, Insect Glycosyltransferases/genetics Mice Molecular Sequence Data Sequence Analysis Sequence Homology, Amino Acid
Chemicals
Glycosyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vollrath B
Howard Hughes Medical Institute and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Fitzgerald K J
Leder P
References (55)
55 references, click to expand
  1. Isolation, characterization and inactivation of the mouse Mgat3 gene: the bisecting N-acetylglucosamine in asparagine-linked oligosaccharides appears dispensable for viability and reproduction.
    Glycobiology. 1997 Feb;7(1):45-56 PMID: 9061364
  2. The neurogenic genes egghead and brainiac define a novel signaling pathway essential for epithelial morphogenesis during Drosophila oogenesis.
    Development. 1996 Dec;122(12):3863-79 PMID: 9012507
  3. Radical fringe positions the apical ectodermal ridge at the dorsoventral boundary of the vertebrate limb.
    Nature. 1997 Mar 27;386(6623):360-6 PMID: 9121551
  4. Expression of Radical fringe in limb-bud ectoderm regulates apical ectodermal ridge formation.
    Nature. 1997 Mar 27;386(6623):366-73 PMID: 9121552
  5. Overexpression of N-acetylglucosaminyltransferase III disrupts the tyrosine phosphorylation of Trk with resultant signaling dysfunction in PC12 cells treated with nerve growth factor.
    J Biol Chem. 1997 Apr 11;272(15):9629-34 PMID: 9092490
  6. Fringe modulates Notch-ligand interactions.
    Nature. 1997 Jun 26;387(6636):908-12 PMID: 9202123
  7. Fringe boundaries coincide with Notch-dependent patterning centres in mammals and alter Notch-dependent development in Drosophila.
    Nat Genet. 1997 Jul;16(3):283-8 PMID: 9207795
  8. Serrate-mediated activation of Notch is specifically blocked by the product of the gene fringe in the dorsal compartment of the Drosophila wing imaginal disc.
    Development. 1997 Aug;124(15):2973-81 PMID: 9247339
  9. The ins and outs of notch signaling.
    Mol Cell Neurosci. 1997;9(2):91-102 PMID: 9245493
  10. Notch signaling in vertebrate development and disease.
    Mol Cell Neurosci. 1997;9(2):103-8 PMID: 9245494
  11. Notch in vertebrates.
    Curr Opin Genet Dev. 1997 Aug;7(4):551-7 PMID: 9309189
  12. Serrate2 is disrupted in the mouse limb-development mutant syndactylism.
    Nature. 1997 Oct 16;389(6652):722-5 PMID: 9338782
  13. Genomic cloning and expression of three murine UDP-galactose: beta-N-acetylglucosamine beta1,3-galactosyltransferase genes.
    J Biol Chem. 1998 Jan 2;273(1):58-65 PMID: 9417047
  14. Defects in limb, craniofacial, and thymic development in Jagged2 mutant mice.
    Genes Dev. 1998 Apr 1;12(7):1046-57 PMID: 9531541
  15. A family of human beta3-galactosyltransferases. Characterization of four members of a UDP-galactose:beta-N-acetyl-glucosamine/beta-nacetyl-galactosamine beta-1,3-galactosyltransferase family.
    J Biol Chem. 1998 May 22;273(21):12770-8 PMID: 9582303
  16. Brainiac and fringe are similar pioneer proteins that impart specificity to notch signaling during Drosophila development.
    Cold Spring Harb Symp Quant Biol. 1997;62:177-84 PMID: 9598350
  17. Nuclear access and action of notch in vivo.
    Cell. 1998 May 15;93(4):649-60 PMID: 9604939
  18. LIN-12/Notch signaling: lessons from worms and flies.
    Genes Dev. 1998 Jun 15;12(12):1751-62 PMID: 9637676
  19. Ligand-induced cleavage and regulation of nuclear entry of Notch in Drosophila melanogaster embryos.
    Genes Dev. 1998 Dec 1;12(23):3728-40 PMID: 9851979
  20. Protein glycosylation in development and disease.
    Bioessays. 1999 May;21(5):412-21 PMID: 10376012
  21. Fringe, Notch, and making developmental boundaries.
    Curr Opin Genet Dev. 1999 Aug;9(4):434-41 PMID: 10449349
  22. Notch1 inhibits neurite outgrowth in postmitotic primary neurons.
    Neuroscience. 1999;93(2):433-9 PMID: 10465425
  23. Notch1 is essential for postimplantation development in mice.
    Genes Dev. 1994 Mar 15;8(6):707-19 PMID: 7926761
  24. Contact-dependent inhibition of cortical neurite growth mediated by notch signaling.
    Science. 1999 Oct 22;286(5440):741-6 PMID: 10531053
  25. Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions.
    Biochim Biophys Acta. 1999 Dec 6;1473(1):35-53 PMID: 10580128
  26. The notch signalling regulator fringe acts in the Golgi apparatus and requires the glycosyltransferase signature motif DXD.
    Curr Biol. 2000 Jul 13;10(14):813-20 PMID: 10899003
  27. Fringe is a glycosyltransferase that modifies Notch.
    Nature. 2000 Jul 27;406(6794):369-75 PMID: 10935626
  28. Glycosyltransferase activity of Fringe modulates Notch-Delta interactions.
    Nature. 2000 Jul 27;406(6794):411-5 PMID: 10935637
  29. Fringe differentially modulates Jagged1 and Delta1 signalling through Notch1 and Notch2.
    Nat Cell Biol. 2000 Aug;2(8):515-20 PMID: 10934472
  30. Identification, expression analysis, and mapping of B3galt6, a putative galactosyl transferase gene with similarity to Drosophila brainiac.
    Mamm Genome. 2001 Feb;12(2):177-9 PMID: 11210191
  31. Induction and repression of mammalian achaete-scute homologue (MASH) gene expression during neuronal differentiation of P19 embryonal carcinoma cells.
    Development. 1992 Jan;114(1):75-87 PMID: 1576967
  32. The involvement of the Notch locus in Drosophila oogenesis.
    Development. 1992 Aug;115(4):913-22 PMID: 1451667
  33. The neurogenic locus brainiac cooperates with the Drosophila EGF receptor to establish the ovarian follicle and to determine its dorsal-ventral polarity.
    Development. 1992 Sep;116(1):177-92 PMID: 1483386
  34. Mice lacking N-acetylglucosaminyltransferase I activity die at mid-gestation, revealing an essential role for complex or hybrid N-linked carbohydrates.
    Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):728-32 PMID: 8290590
  35. Complex asparagine-linked oligosaccharides are required for morphogenic events during post-implantation development.
    EMBO J. 1994 May 1;13(9):2056-65 PMID: 8187759
  36. fringe, a Boundary-specific signaling molecule, mediates interactions between dorsal and ventral cells during Drosophila wing development.
    Cell. 1994 Nov 18;79(4):595-606 PMID: 7954826
  37. An activated Notch suppresses neurogenesis and myogenesis but not gliogenesis in mammalian cells.
    Development. 1994 Sep;120(9):2421-30 PMID: 7956822
  38. Murine FGFR-1 is required for early postimplantation growth and axial organization.
    Genes Dev. 1994 Dec 15;8(24):3045-57 PMID: 8001823
  39. The Notch signalling pathway is required for Enhancer of split bHLH protein expression during neurogenesis in the Drosophila embryo.
    Development. 1994 Dec;120(12):3537-48 PMID: 7821220
  40. Notch signaling.
    Science. 1995 Apr 14;268(5208):225-32 PMID: 7716513
  41. Suppression of lung metastasis of B16 mouse melanoma by N-acetylglucosaminyltransferase III gene transfection.
    Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8754-8 PMID: 7568011
  42. The neurogenic suppressor of hairless DNA-binding protein mediates the transcriptional activation of the enhancer of split complex genes triggered by Notch signaling.
    Genes Dev. 1995 Nov 1;9(21):2598-608 PMID: 7590238
  43. Suppressor of hairless directly activates transcription of enhancer of split complex genes in response to Notch receptor activity.
    Genes Dev. 1995 Nov 1;9(21):2609-22 PMID: 7590239
  44. Complex asparagine-linked oligosaccharides in Mgat1-null embryos.
    Glycobiology. 1995 Jul;5(5):535-43 PMID: 8563140
  45. Targeted disruption of mammalian hairy and Enhancer of split homolog-1 (HES-1) leads to up-regulation of neural helix-loop-helix factors, premature neurogenesis, and severe neural tube defects.
    Genes Dev. 1995 Dec 15;9(24):3136-48 PMID: 8543157
  46. Involvement of Notch-1 in mammalian retinal neurogenesis: association of Notch-1 activity with both immature and terminally differentiated cells.
    Mech Dev. 1995 Sep;53(1):73-85 PMID: 8555113
  47. Fibroblast growth factor receptor 3 is a negative regulator of bone growth.
    Cell. 1996 Mar 22;84(6):911-21 PMID: 8601314
  48. Notch-related genes in animal development.
    Int J Dev Biol. 1995 Oct;39(5):769-80 PMID: 8645561
  49. Aberrant glycosylation of E-cadherin enhances cell-cell binding to suppress metastasis.
    J Biol Chem. 1996 Jun 7;271(23):13811-5 PMID: 8662832
  50. Functional relationships between Notch, Su(H) and the bHLH genes of the E(spl) complex: the E(spl) genes mediate only a subset of Notch activities during imaginal development.
    Development. 1996 Sep;122(9):2719-28 PMID: 8787746
  51. Expression patterns of Jagged, Delta1, Notch1, Notch2, and Notch3 genes identify ligand-receptor pairs that may function in neural development.
    Mol Cell Neurosci. 1996;8(1):14-27 PMID: 8923452
  52. Jagged2: a serrate-like gene expressed during rat embryogenesis.
    Dev Biol. 1996 Nov 25;180(1):370-6 PMID: 8948600
  53. Secreted fringe-like signaling molecules may be glycosyltransferases.
    Cell. 1997 Jan 10;88(1):9-11 PMID: 9019410
  54. The expression and function of Notch pathway genes in the developing rat eye.
    J Neurosci. 1997 Feb 15;17(4):1425-34 PMID: 9006984
  55. Brainiac encodes a novel, putative secreted protein that cooperates with Grk TGF alpha in the genesis of the follicular epithelium.
    Dev Biol. 1996 Aug 25;178(1):35-50 PMID: 8812107
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.16.5688-5697.2001
Published
2001-08-00
Pages
5688-97
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87289
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.21.16.5688-5697.2001 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2