ULBP1, 2, 3: novel MHC class I-related molecules that bind to human cytomegalovirus glycoprotein UL16, activate NK cells.

European journal of immunology ·Vol. 31 ·No. 5 ·2001-05-00 ·Pages 1428-37

Kubin M, Cassiano L, Chalupny J, Chin W, Cosman D, Fanslow W, Müllberg J, Rousseau AM, Ulrich D, Armitage R

Abstract

New members of the extended MHC class I-like family were identified based on their ability to bind human cytomegalovirus glycoprotein UL16 and/or their mutual homology. Soluble UL16 binding proteins (ULBP) competed with each other for binding to NK cells. Treatment of human and mouse NK cells with ULBP led to increased production of cytokines/chemokines, proliferation, cytotoxic activity and up-regulation of activation-associated surface molecules. The presence of ULBP during the stimulation phase of the CTL assay caused increased cytotoxic activity. Addition of soluble recombinant UL16 protein inhibited the biological activities mediated by ULBP, suggesting the existence of a novel mechanism utilized by CMV to evade elimination by the host immune system.

MeSH Terms

Animals Binding, Competitive Carrier Proteins/antagonists & inhibitors,metabolism Cell Division/drug effects Cells, Cultured Chemokines/metabolism Cytokines/metabolism Cytomegalovirus/immunology,metabolism Cytotoxicity, Immunologic Flow Cytometry GPI-Linked Proteins Histocompatibility Antigens Class I/metabolism Humans Immunoglobulin G/immunology Intercellular Signaling Peptides and Proteins Interleukin-15/immunology Intracellular Signaling Peptides and Proteins Killer Cells, Natural/cytology,drug effects,immunology,metabolism Lymphocyte Activation/drug effects Lymphocyte Count Membrane Proteins Mice Mice, SCID Protein Binding Solubility Spleen/cytology T-Lymphocytes, Cytotoxic/immunology Viral Envelope Proteins/metabolism,pharmacology

Chemicals

Carrier Proteins Chemokines Cytokines GPI-Linked Proteins Histocompatibility Antigens Class I Immunoglobulin G Intercellular Signaling Peptides and Proteins Interleukin-15 Intracellular Signaling Peptides and Proteins Membrane Proteins ULBP1 protein, human ULBP2 protein, human ULBP3 protein, human Viral Envelope Proteins

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Kubin M

Department of Immunobiology, Immunex Corporation, Seattle 98101, USA. [email protected]

Cassiano L

Chalupny J

Chin W

Cosman D

Fanslow W

Müllberg J

Rousseau A M

Ulrich D

Armitage R

Article Info

Journal

European journal of immunology

Abbr.

Eur J Immunol

ISSN

0014-2980

DOI

10.1002/1521-4141(200105)31:5<1428::AID-IMMU1428>3.0.CO;2-4

[email protected]

Published

2001-05-00

Pages

1428-37

Language

English

Region

Germany

NLM ID

1273201

Subset

External Links

PubMed source DOI: 10.1002/1521-4141(200105)31:5<1428::AID-IMMU1428>3.0.CO;2-4 View journal details

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ULBP1, 2, 3: novel MHC class I-related molecules that bind to human cytomegalovirus glycoprotein UL16, activate NK cells.

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