Modification of the template capacity of liver chromatin for form-B ribonucleic acid polymerase by food intake in rats under controlled feeding schedules.

The Biochemical journal ·Vol. 146 ·No. 3 ·1975-03-00 ·Pages 687-96

Barbiroli B, Tadolini B, Moruzzi MS, Monti MG

Abstract

Nuclei from liver of rats accustomed to eating during the first 8h of a daily 12h dark period demonstrate an increased capacity to synthesize RNA 6H after the beginning of the feeding period. 2. This increase is accompanied by a higher yield of extractable form-B DNA-dependent RNA polymerase activity. 3. The endogenous RNA polymerase activity associated with nuclear chromatin is also stimulated by food intake. Both purified and chromatin-associated form-B enzyme activities exhibit different ionic strength requirements after food intake. 4. The sensitivity of exogenous (added) form-B-enzyme to changes in ionic strength changes after feeding when chromatin is used as template. 5. Chromatin extracted from the liver of fed rats is a better template for form-B-enzyme than chromatin extracted from starved rats.

MeSH Terms

Ammonium Sulfate Animals Chromatin/metabolism DNA DNA-Directed RNA Polymerases/metabolism Food Kinetics Liver/metabolism Male Osmolar Concentration RNA/analysis,biosynthesis Rats Templates, Genetic Time Factors

Chemicals

Chromatin RNA DNA DNA-Directed RNA Polymerases Ammonium Sulfate

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Barbiroli B

Tadolini B

Moruzzi M S

Monti M G

References (28)

28 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1460687

Published

1975-03-00

Pages

687-96

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1165359

Subset

External Links

PubMed source DOI: 10.1042/bj1460687 PMC Full Text View journal details

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Modification of the template capacity of liver chromatin for form-B ribonucleic acid polymerase by food intake in rats under controlled feeding schedules.

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