The kamikaze approach to membrane transport.

Nature reviews. Molecular cell biology ·Vol. 2 ·No. 8 ·2001-08-00 ·Pages 610-20

Kaback HR, Sahin-Tóth M, Weinglass AB

Abstract

Membrane transport proteins catalyse the movement of molecules into and out of cells and organelles, but their hydrophobic and metastable nature often makes them difficult to study by traditional means. Novel approaches that have been developed and applied to one membrane transport protein, the lactose permease from Escherichia coli, are now being used to study various other membrane proteins.

MeSH Terms

Alkylation Amino Acid Sequence Bacterial Proteins/chemistry,genetics,physiology Binding Sites Biological Transport/physiology Carrier Proteins/physiology Cysteine/chemistry Escherichia coli/enzymology,genetics Escherichia coli Proteins Ethylmaleimide/pharmacology Humans Lactose/metabolism Long QT Syndrome/metabolism Membrane Proteins/physiology Membrane Transport Proteins/chemistry,genetics,physiology Metabolism, Inborn Errors/metabolism Models, Molecular Molecular Sequence Data Monosaccharide Transport Proteins Mutagenesis, Site-Directed Protein Conformation Protons Recombinant Fusion Proteins/metabolism Structure-Activity Relationship Sulfhydryl Reagents/pharmacology Symporters

Chemicals

Bacterial Proteins Carrier Proteins Escherichia coli Proteins LacY protein, E coli Membrane Proteins Membrane Transport Proteins Monosaccharide Transport Proteins Protons Recombinant Fusion Proteins Sulfhydryl Reagents Symporters lactose permease Lactose Cysteine Ethylmaleimide

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kaback H R

Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles, California 90095-1662, USA. [email protected]

Sahin-Tóth M

Weinglass A B

Article Info

Journal

Nature reviews. Molecular cell biology

Abbr.

Nat Rev Mol Cell Biol

ISSN

1471-0072

DOI

10.1038/35085077

[email protected]

Published

2001-08-00

Pages

610-20

Language

English

Region

England

NLM ID

100962782

Subset

External Links

PubMed source DOI: 10.1038/35085077 View journal details

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The kamikaze approach to membrane transport.

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