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PMID: 11485162 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of dietary restriction on age-related increase of liver susceptibility to peroxidation in rats.

Lipids ·Vol. 36 ·No. 6 ·2001-06-00 ·Pages 589-93

Jeon TI, Lim BO, Yu BP, Lim Y, Jeon EJ, Park DK

Abstract

Dietary restriction (DR) increases life span and decreases age-related diseases in experimental animals. It has received a great deal of attention in connection with the relationship between aging, nutrition, and oxidative stress because oxidative injury in several organ systems is a prominent feature in aging. We investigated the possibility that DR can protect vulnerable liver lipids against age-related increases of peroxidation. Male Fischer 344 rats fed ad libitum (AL) or dietarily restricted (maintained on 60% of AL food intake) were killed by decapitation at 4 (young) or 12 mon (adult) of age. Phosphatidylcholine hydroperoxide (PCOOH) concentration of liver was determined using a chemiluminescent high-performance liquid chromatographic method. Liver PCOOH increased with age in adult rats, but less of an increase of PCOOH was seen in DR rats, which is consistent with results on production of thiobarbituric acid-reactive substances and oxygen-derived free radicals. No significant differences were found in liver superoxide dismutase and catalase activity between AL and DR groups of young and adult rats. Liver triglyceride and cholesterol contents were lower in DR than AL rats at 12 mon. Fatty acid compositions of phosphatidylcholine and phosphatidylethanolamine indicated that the ratio of (20:3n-6 + 20:4n-6)/18:2n-6, an index of linoleic acid (18:2n-6) desaturation, was lower in DR than in AL rats. We concluded that DR suppresses age-related oxidative damage in liver by modulating the amount of lipid as well as fatty acid composition.

MeSH Terms
Aging/physiology Animals Body Weight Catalase/metabolism Chromatography, High Pressure Liquid/methods Diet, Reducing Hydrogen Peroxide/metabolism Lipid Peroxidation/physiology Liver/metabolism Luminescent Measurements Male Oxidative Stress Phosphatidylcholines/chemistry,metabolism Phosphatidylethanolamines/chemistry,metabolism Phospholipids/analysis,metabolism Rats Rats, Inbred F344 Superoxide Dismutase/metabolism
Chemicals
Phosphatidylcholines Phosphatidylethanolamines Phospholipids Hydrogen Peroxide Catalase Superoxide Dismutase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jeon T I
Department of Applied Biology and Chemistry, Konkuk University, Seoul, Korea.
Lim B O
Yu B P
Lim Y
Jeon E J
Park D K
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Article Info
Journal
Lipids
Abbr.
Lipids
ISSN
0024-4201
DOI
10.1007/s11745-001-0761-1
Published
2001-06-00
Pages
589-93
Language
English
Region
United States
NLM ID
0060450
Subset
IM
External Links
PubMed source DOI: 10.1007/s11745-001-0761-1 View journal details
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