Home LiteratureArticle Details
PMID: 11511535 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of cyclin D2 gene expression by the Myc/Max/Mad network: Myc-dependent TRRAP recruitment and histone acetylation at the cyclin D2 promoter.

Genes & development ·Vol. 15 ·No. 16 ·2001-08-15 ·Pages 2042-7

Bouchard C, Dittrich O, Kiermaier A, Dohmann K, Menkel A, Eilers M, Lüscher B

Abstract

Myc oncoproteins promote cell cycle progression in part through the transcriptional up-regulation of the cyclin D2 gene. We now show that Myc is bound to the cyclin D2 promoter in vivo. Binding of Myc induces cyclin D2 expression and histone acetylation at a single nucleosome in a MycBoxII/TRRAP-dependent manner. Down-regulation of cyclin D2 mRNA expression in differentiating HL60 cells is preceded by a switch of promoter occupancy from Myc/Max to Mad/Max complexes, loss of TRRAP binding, increased HDAC1 binding, and histone deacetylation. Thus, recruitment of TRRAP and regulation of histone acetylation are critical for transcriptional activation by Myc.

MeSH Terms
Acetylation Bacterial Proteins Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Basic-Leucine Zipper Transcription Factors Cyclin D2 Cyclins/genetics DNA-Binding Proteins/physiology Gene Expression Regulation HL-60 Cells Histones/metabolism Humans Promoter Regions, Genetic/physiology Proto-Oncogene Proteins c-myc/physiology RNA, Messenger/biosynthesis RNA-Binding Proteins/physiology Repressor Proteins Transcription Factors/physiology
Chemicals
Bacterial Proteins Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Basic-Leucine Zipper Transcription Factors CCND2 protein, human Cyclin D2 Cyclins DNA-Binding Proteins Histones MAX protein, human MXD1 protein, human MtrB protein, Bacteria Myc associated factor X Proto-Oncogene Proteins c-myc RNA, Messenger RNA-Binding Proteins Repressor Proteins Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bouchard C
Institute for Molecular Biology and Tumor Research, 35033 Marburg, Germany.
Dittrich O
Kiermaier A
Dohmann K
Menkel A
Eilers M
Lüscher B
References (28)
28 references, click to expand
  1. Histone deacetylases associated with the mSin3 corepressor mediate mad transcriptional repression.
    Cell. 1997 May 2;89(3):349-56 PMID: 9150134
  2. Histone deacetylase activity is required for full transcriptional repression by mSin3A.
    Cell. 1997 May 2;89(3):341-7 PMID: 9150133
  3. Analysis of the DNA-binding activities of Myc/Max/Mad network complexes during induced differentiation of U-937 monoblasts and F9 teratocarcinoma cells.
    Oncogene. 1997 Aug 7;15(6):737-48 PMID: 9264414
  4. Identification and characterization of specific DNA-binding complexes containing members of the Myc/Max/Mad network of transcriptional regulators.
    J Biol Chem. 1998 Mar 20;273(12):6632-42 PMID: 9506959
  5. The novel ATM-related protein TRRAP is an essential cofactor for the c-Myc and E2F oncoproteins.
    Cell. 1998 Aug 7;94(3):363-74 PMID: 9708738
  6. Transactivation-defective c-MycS retains the ability to regulate proliferation and apoptosis.
    Genes Dev. 1998 Dec 15;12(24):3803-8 PMID: 9869633
  7. The ATM-related cofactor Tra1 is a component of the purified SAGA complex.
    Mol Cell. 1998 Dec;2(6):863-7 PMID: 9885573
  8. c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function.
    Nat Genet. 1999 May;22(1):102-5 PMID: 10319872
  9. Direct induction of cyclin D2 by Myc contributes to cell cycle progression and sequestration of p27.
    EMBO J. 1999 Oct 1;18(19):5321-33 PMID: 10508165
  10. The essential cofactor TRRAP recruits the histone acetyltransferase hGCN5 to c-Myc.
    Mol Cell Biol. 2000 Jan;20(2):556-62 PMID: 10611234
  11. Gene-target recognition among members of the myc superfamily and implications for oncogenesis.
    Nat Genet. 2000 Feb;24(2):113-9 PMID: 10655054
  12. Identification of CDK4 as a target of c-MYC.
    Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2229-34 PMID: 10688915
  13. Expression analysis with oligonucleotide microarrays reveals that MYC regulates genes involved in growth, cell cycle, signaling, and adhesion.
    Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3260-5 PMID: 10737792
  14. Mapping DNA target sites of chromatin proteins in vivo by formaldehyde crosslinking.
    Methods Mol Biol. 1999;119:455-67 PMID: 10804532
  15. Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis.
    Cell. 2000 Aug 18;102(4):463-73 PMID: 10966108
  16. Myc-enhanced expression of Cul1 promotes ubiquitin-dependent proteolysis and cell cycle progression.
    Genes Dev. 2000 Sep 1;14(17):2185-91 PMID: 10970882
  17. The Myc/Max/Mad network and the transcriptional control of cell behavior.
    Annu Rev Cell Dev Biol. 2000;16:653-99 PMID: 11031250
  18. Direct examination of histone acetylation on Myc target genes using chromatin immunoprecipitation.
    J Biol Chem. 2000 Oct 27;275(43):33798-805 PMID: 10931841
  19. Differential activity of conditional MYC and its variant MYC-S in human mortal fibroblasts.
    Oncogene. 2000 Oct 26;19(45):5189-97 PMID: 11064456
  20. Colony-stimulating factor-1 receptor utilizes multiple signaling pathways to induce cyclin D2 expression.
    Mol Biol Cell. 2000 Nov;11(11):3835-48 PMID: 11071910
  21. Identification of c-myc responsive genes using rat cDNA microarray.
    Cancer Res. 2000 Nov 1;60(21):5922-8 PMID: 11085504
  22. Function of the c-Myc oncoprotein in chromatin remodeling and transcription.
    Biochim Biophys Acta. 2001 Mar 21;1471(3):M135-45 PMID: 11250069
  23. Switch from Myc/Max to Mad1/Max binding and decrease in histone acetylation at the telomerase reverse transcriptase promoter during differentiation of HL60 cells.
    Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3826-31 PMID: 11274400
  24. Dominant negative MYC blocks transformation by ABL oncogenes.
    Cell. 1992 Sep 18;70(6):901-10 PMID: 1525828
  25. A switch from Myc:Max to Mad:Max heterocomplexes accompanies monocyte/macrophage differentiation.
    Genes Dev. 1993 Nov;7(11):2110-9 PMID: 8224841
  26. Mad proteins contain a dominant transcription repression domain.
    Mol Cell Biol. 1996 Oct;16(10):5772-81 PMID: 8816491
  27. Role for N-CoR and histone deacetylase in Sin3-mediated transcriptional repression.
    Nature. 1997 May 1;387(6628):49-55 PMID: 9139821
  28. Cell growth inhibition by the Mad/Max complex through recruitment of histone deacetylase activity.
    Curr Biol. 1997 Jun 1;7(6):357-65 PMID: 9197243
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.907901
Published
2001-08-15
Pages
2042-7
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312761
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.907901 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2