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PMID: 11544178 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Large scale transgenic and cluster deletion analysis of the HoxD complex separate an ancestral regulatory module from evolutionary innovations.

Genes & development ·Vol. 15 ·No. 17 ·2001-09-01 ·Pages 2209-14

Spitz F, Gonzalez F, Peichel C, Vogt TF, Duboule D, Zákány J

Abstract

The ancestral role of the Hox gene family is specifying morphogenetic differences along the main body axis. In vertebrates, HoxD genes were also co-opted along with the emergence of novel structures such as limbs and genitalia. We propose that these functional recruitments relied on the appearance, or implementation, of regulatory sequences outside of the complex. Whereas transgenic human and murine HOXD clusters could function during axial patterning, in mice they were not expressed outside the trunk. Accordingly, deletion of the entire cluster abolished axial expression, whereas recently acquired regulatory controls were preserved.

MeSH Terms
Animals Bone Development/genetics DNA-Binding Proteins Embryo, Mammalian/metabolism Evolution, Molecular Gene Deletion Gene Expression Regulation, Developmental Genes, Reporter Homeodomain Proteins/genetics,physiology Humans In Situ Hybridization Mice Mice, Inbred C57BL Mice, Inbred DBA Mice, Transgenic Models, Genetic Multigene Family Mutation Neoplasm Proteins Phenotype Recombination, Genetic Time Factors Transcription Factors/genetics
Chemicals
DNA-Binding Proteins HOXD13 protein, human Homeodomain Proteins Hoxd13 protein, mouse Hoxd3 protein, mouse Hoxd4 protein, mouse Hoxd8 protein, mouse Hoxd9 protein, mouse Neoplasm Proteins Transcription Factors HOXA4 protein, human HOXD10 protein, human HOXD8 protein, human
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Spitz F
Department of Zoology and Animal Biology, University of Geneva, Sciences III, 1211 Geneva 4, Switzerland.
Gonzalez F
Peichel C
Vogt T F
Duboule D
Zákány J
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.205701
Published
2001-09-01
Pages
2209-14
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312772
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.205701 PMC Full Text View journal details
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