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PMID: 11554480 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A census of carbohydrate-active enzymes in the genome of Arabidopsis thaliana.

Plant molecular biology ·Vol. 47 ·No. 1-2 ·2001-09-00 ·Pages 55-72

Henrissat B, Coutinho PM, Davies GJ

Abstract

The synthesis, modification, and breakdown of carbohydrates is one of the most fundamentally important reactions in nature. The structural and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdown (glycoside hydrolases and polysaccharide lyases). The importance of these processes is reflected in the dedication of 1-2% of an organism's genes to glycoside hydrolases and glycosyltransferases alone. In plants, these processes are of particular importance for cell-wall synthesis and expansion. starch metabolism, defence against pathogens, symbiosis and signalling. Here we present an analysis of over 730 open reading frames representing the two main classes of carbohydrate-active enzymes, glycoside hydrolases and glycosyltransferases, in the genome of Arabidopsis thaliana. The vast importance of these enzymes in cell-wall formation and degradation is revealed along with the unexpected dominance of pectin degradation in Arabidopsis, with at least 170 open-reading frames dedicated solely to this task.

MeSH Terms
Arabidopsis/enzymology,genetics,metabolism Carbohydrate Metabolism Cell Wall/metabolism Enzymes/chemistry,genetics,metabolism Genome, Plant Glycoside Hydrolases/chemistry,genetics,metabolism Glycosyltransferases/chemistry,genetics,metabolism Open Reading Frames Protein Conformation
Chemicals
Enzymes Glycosyltransferases Glycoside Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Henrissat B
Architecture et Fonction des Macromolécules Biologiques, UMR 6098, CNRS and Universités d'Aix-Marseille I and II, France. [email protected]
Coutinho P M
Davies G J
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
Email
[email protected]
Published
2001-09-00
Pages
55-72
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source View journal details
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