Home LiteratureArticle Details
PMID: 11571165 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Relationships between microbial community structure and hydrochemistry in a landfill leachate-polluted aquifer.

Applied and environmental microbiology ·Vol. 67 ·No. 10 ·2001-10-00 ·Pages 4619-29

Röling WF, van Breukelen BM, Braster M, Lin B, van Verseveld HW

Abstract

Knowledge about the relationship between microbial community structure and hydrogeochemistry (e.g., pollution, redox and degradation processes) in landfill leachate-polluted aquifers is required to develop tools for predicting and monitoring natural attenuation. In this study analyses of pollutant and redox chemistry were conducted in parallel with culture-independent profiling of microbial communities present in a well-defined aquifer (Banisveld, The Netherlands). Degradation of organic contaminants occurred under iron-reducing conditions in the plume of pollution, while upstream of the landfill and above the plume denitrification was the dominant redox process. Beneath the plume iron reduction occurred. Numerical comparison of 16S ribosomal DNA (rDNA)-based denaturing gradient gel electrophoresis (DGGE) profiles of Bacteria and Archaea in 29 groundwater samples revealed a clear difference between the microbial community structures inside and outside the contaminant plume. A similar relationship was not evident in sediment samples. DGGE data were supported by sequencing cloned 16S rDNA. Upstream of the landfill members of the beta subclass of the class Proteobacteria (beta-proteobacteria) dominated. This group was not encountered beneath the landfill, where gram-positive bacteria dominated. Further downstream the contribution of gram-positive bacteria to the clone library decreased, while the contribution of delta-proteobacteria strongly increased and beta-proteobacteria reappeared. The beta-proteobacteria (Acidovorax, Rhodoferax) differed considerably from those found upstream (Gallionella, Azoarcus). Direct comparisons of cloned 16S rDNA with bands in DGGE profiles revealed that the data from each analysis were comparable. A relationship was observed between the dominant redox processes and the bacteria identified. In the iron-reducing plume members of the family Geobacteraceae made a strong contribution to the microbial communities. Because the only known aromatic hydrocarbon-degrading, iron-reducing bacteria are Geobacter spp., their occurrence in landfill leachate-contaminated aquifers deserves more detailed consideration.

MeSH Terms
Archaea/classification,genetics Bacteria/classification,genetics Cloning, Molecular DNA, Ribosomal/analysis Ecosystem Electrophoresis, Polyacrylamide Gel/methods Fresh Water/chemistry,microbiology Geologic Sediments/chemistry,microbiology Molecular Sequence Data RNA, Ribosomal, 16S/genetics Refuse Disposal Sequence Analysis, DNA Water Pollution, Chemical
Chemicals
DNA, Ribosomal RNA, Ribosomal, 16S
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Röling W F
Section of Molecular Microbial Ecology, Department of Molecular Cell Physiology, Faculty of Biology, Research School SENSE, Vrije Universiteit, NL-1081 HV Amsterdam, The Netherlands.
van Breukelen B M
Braster M
Lin B
van Verseveld H W
References (30)
30 references, click to expand
  1. Geothrix fermentans gen. nov., sp. nov., a novel Fe(III)-reducing bacterium from a hydrocarbon-contaminated aquifer.
    Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1615-22 PMID: 10555343
  2. Microbial communities associated with anaerobic benzene degradation in a petroleum-contaminated aquifer.
    Appl Environ Microbiol. 1999 Jul;65(7):3056-63 PMID: 10388703
  3. Analysis of Microbial Communities in a Landfill Leachate Polluted Aquifer using a New Method for Anaerobic Physiological Profiling and 16S rDNA Based Fingerprinting.
    Microb Ecol. 2000 Aug;40(3):177-188 PMID: 11080376
  4. Oxidation capacity of aquifer sediments.
    Environ Sci Technol. 1994 Jan 1;28(1):153-8 PMID: 22175845
  5. Phylogenetic analyses of a new group of denitrifiers capable of anaerobic growth of toluene and description of Azoarcus tolulyticus sp. nov.
    Int J Syst Bacteriol. 1995 Jul;45(3):500-6 PMID: 8590677
  6. Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA.
    Appl Environ Microbiol. 1997 Sep;63(9):3367-73 PMID: 9292986
  7. Phylogeny of the main bacterial 16S rRNA sequences in Drentse A grassland soils (The Netherlands).
    Appl Environ Microbiol. 1998 Mar;64(3):871-9 PMID: 9501427
  8. Prokaryotes: the unseen majority.
    Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6578-83 PMID: 9618454
  9. Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.
    Appl Environ Microbiol. 1992 Feb;58(2):614-23 PMID: 1376982
  10. Increase in bacterial community diversity in subsurface aquifers receiving livestock wastewater input.
    Appl Environ Microbiol. 2000 Mar;66(3):956-65 PMID: 10698758
  11. Isolation of Geobacter species from diverse sedimentary environments.
    Appl Environ Microbiol. 1996 May;62(5):1531-6 PMID: 8633852
  12. Bioremediation of organic and metal contaminants with dissimilatory metal reduction.
    J Ind Microbiol. 1995 Feb;14(2):85-93 PMID: 7766214
  13. Protistan communities in aquifers: a review.
    FEMS Microbiol Rev. 1997 Jul;20(3-4):261-75 PMID: 9299706
  14. Distribution and Composition of Microbial Populations in a Landfill Leachate Contaminated Aquifer (Grindsted, Denmark).
    Microb Ecol. 1999 Apr;37(3):197-207 PMID: 10227877
  15. Immunochemical detection and isolation of DNA from metabolically active bacteria.
    Appl Environ Microbiol. 1999 Mar;65(3):1207-13 PMID: 10049885
  16. Effects of agronomic treatments on structure and function of ammonia-oxidizing communities.
    Appl Environ Microbiol. 2000 Dec;66(12):5410-8 PMID: 11097922
  17. Phylogenetic analysis of particle-attached and free-living bacterial communities in the Columbia river, its estuary, and the adjacent coastal ocean.
    Appl Environ Microbiol. 1999 Jul;65(7):3192-204 PMID: 10388721
  18. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  19. Bacterial population development and chemical characteristics of refuse decomposition in a simulated sanitary landfill.
    Appl Environ Microbiol. 1989 Jan;55(1):55-65 PMID: 16347835
  20. Stable-isotope probing as a tool in microbial ecology.
    Nature. 2000 Feb 10;403(6770):646-9 PMID: 10688198
  21. Enrichment of Geobacter Species in Response to Stimulation of Fe(III) Reduction in Sandy Aquifer Sediments.
    Microb Ecol. 2000 Feb;39(2):153-167 PMID: 10833228
  22. Recovery of humic-reducing bacteria from a diversity of environments.
    Appl Environ Microbiol. 1998 Apr;64(4):1504-9 PMID: 9546186
  23. Microbial biomass and activity in subsurface sediments from Vejen, Denmark.
    Microb Ecol. 1992 May;23(3):303-17 PMID: 24192937
  24. Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis.
    FEMS Microbiol Rev. 1997 Nov;21(3):213-29 PMID: 9451814
  25. Geovibrio ferrireducens, a phylogenetically distinct dissimilatory Fe(III)-reducing bacterium.
    Arch Microbiol. 1996 Jun;165(6):370-6 PMID: 8661930
  26. Distribution of organic compounds from municipal solid waste in the groundwater downgradient of a landfill (grindsted, denmark).
    Environ Sci Technol. 1995 May 1;29(5):1395-400 PMID: 22192038
  27. Phylogenetic analysis of dissimilatory Fe(III)-reducing bacteria.
    J Bacteriol. 1996 Apr;178(8):2402-8 PMID: 8636045
  28. Importance of unattached bacteria and bacteria attached to sediment in determining potentials for degradation of xenobiotic organic contaminants in an aerobic aquifer.
    Appl Environ Microbiol. 1992 Sep;58(9):3020-6 PMID: 1444415
  29. A molecular view of microbial diversity in a dynamic landfill in Québec.
    FEMS Microbiol Lett. 1998 May 15;162(2):219-26 PMID: 9627956
  30. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
    Appl Environ Microbiol. 1993 Mar;59(3):695-700 PMID: 7683183
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.67.10.4619-4629.2001
Published
2001-10-00
Pages
4619-29
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC93212
Subset
IM
Databases
GENBANK
AY013585, AY013586, AY013587, AY013588, AY013589, AY013590, AY013591, AY013592, AY013593, AY013594, AY013595, AY013596, AY013597, AY013598, AY013599, AY013600, AY013601, AY013602, AY013603, AY013604, AY013605, AY013606, AY013607, AY013608, AY013609, AY013610, AY013611, AY013612, AY013613, AY013614, AY013615, AY013616, AY013617, AY013618, AY013619, AY013620, AY013621, AY013622, AY013623, AY013624, AY013625, AY013626, AY013627, AY013628, AY013629, AY013630, AY013631, AY013632, AY013633, AY013634, AY013635, AY013636, AY013637, AY013638, AY013639, AY013640, AY013641, AY013642, AY013643, AY013644, AY013645, AY013646, AY013647, AY013648, AY013649, AY013650, AY013651, AY013652, AY013653, AY013654, AY013655, AY013656, AY013657, AY013658, AY013660, AY013661, AY013662, AY013663, AY013664, AY013665, AY013666, AY013667, AY013668, AY013669, AY013670, AY013671, AY013672, AY013673, AY013674, AY013675, AY013676, AY013677, AY013678, AY013679, AY013680, AY013681, AY013682, AY013683, AY013684, AY013685, AY013686, AY013687, AY013688, AY013689, AY013690, AY013691, AY013692, AY013693, AY013694, AY013695, AY013696, AY013697, AY013698
External Links
PubMed source DOI: 10.1128/AEM.67.10.4619-4629.2001 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2