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PMID: 11571197 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Axial differences in community structure of Crenarchaeota and Euryarchaeota in the highly compartmentalized gut of the soil-feeding termite Cubitermes orthognathus.

Applied and environmental microbiology ·Vol. 67 ·No. 10 ·2001-10-00 ·Pages 4880-90

Friedrich MW, Schmitt-Wagner D, Lueders T, Brune A

Abstract

Methanogenesis represents an important electron sink reaction in the hindgut of soil-feeding termites. This is the first comprehensive analysis of the archaeal community structure within the highly compartmentalized intestinal tract of a humivorous insect, combining clonal analysis and terminal restriction fragment (T-RF) length polymorphism (T-RFLP) fingerprinting of the archaeal communities in the different gut compartments of Cubitermes orthognathus. We found that the morphological and physicochemical heterogeneity of the gut is reflected in a large phylogenetic diversity and pronounced axial differences in the composition of the archaeal gut microbiota, notably among those clones or ribotypes that could be assigned to methanogenic taxa. Comparative analysis of the relative frequencies of different archaeal lineages among the small-subunit rRNA gene (SSU rDNA) clones and their corresponding T-RF indicated that the archaeal community in the anterior, extremely alkaline hindgut compartment (P1) consists mainly of members of the Methanosarcinaceae, whereas Methanobacteriaceae and Methanomicrobiales predominate in the subsequent, more posterior compartments (P3/4a and P4b). The relative abundance of Thermoplasmales increased towards the rectum (P5). SSU rDNA sequences representing Crenarchaeota, which have not yet been reported to occur in the intestinal tracts of arthropods, were detected in all gut sections. We discuss how the spatial distribution of methanogenic populations may be linked to axial heterogeneity in the physicochemical gut conditions and to functional adaptations to their respective ecological niches.

MeSH Terms
Animals Crenarchaeota/classification,genetics DNA, Archaeal/analysis Ecosystem Euryarchaeota/classification,genetics Genes, rRNA Intestines/microbiology Isoptera/microbiology,physiology Molecular Sequence Data Phylogeny Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Soil
Chemicals
DNA, Archaeal RNA, Ribosomal, 16S Soil
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Friedrich M W
Max-Planck-Institute for Terrestrial Microbiology, D-35043 Marburg/Lahn, Germany. [email protected]
Schmitt-Wagner D
Lueders T
Brune A
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.67.10.4880-4890.2001
Email
[email protected]
Published
2001-10-00
Pages
4880-90
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC93244
Subset
IM
Databases
GENBANK
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External Links
PubMed source DOI: 10.1128/AEM.67.10.4880-4890.2001 PMC Full Text View journal details
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