Home LiteratureArticle Details
PMID: 11571208 Published · ppublish English Comparative Study Journal Article

Comparative study of bacterial groups within the human cecal and fecal microbiota.

Applied and environmental microbiology ·Vol. 67 ·No. 10 ·2001-10-00 ·Pages 4939-42

Marteau P, Pochart P, Doré J, Béra-Maillet C, Bernalier A, Corthier G

Abstract

The composition of the human cecal microbiota is poorly known because of sampling difficulties. Samples of cecal fluid from eight subjects were collected via an intestinal tube. Feces were also collected. Total anaerobes, facultative anaerobes, bifidobacteria, and Bacteroides were enumerated by culture methods, and the predominant phylogenetic groups were quantified by molecular hybridization using a set of six rRNA-targeted probes. The numbers of strict anaerobes, bifidobacteria, Bacteroides, and members of the Clostridium coccoides group and Clostridium leptum subgroup were lower in the cecum. Facultative anaerobes represented 25% of total bacteria in the cecum versus 1% in the feces.

MeSH Terms
Adult Bacteria, Anaerobic/classification,isolation & purification Bacteroides/classification,isolation & purification Bifidobacterium/classification,isolation & purification Cecum/microbiology Clostridium/classification,isolation & purification Culture Media Feces/microbiology Female Humans Male RNA, Ribosomal, 16S/genetics
Chemicals
Culture Media RNA, Ribosomal, 16S
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Marteau P
INSERM U 290, Paris, France. [email protected]
Pochart P
Doré J
Béra-Maillet C
Bernalier A
Corthier G
References (22)
22 references, click to expand
  1. Physicochemical environment of the colon.
    Eur J Cancer Prev. 1998 May;7 Suppl 2:S79-80 PMID: 9696947
  2. Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies.
    Appl Environ Microbiol. 1996 Dec;62(12):4504-13 PMID: 8953722
  3. Design and evaluation of a 16S rRNA-targeted oligonucleotide probe for specific detection and quantitation of human faecal Bacteroides populations.
    Syst Appl Microbiol. 1998 Mar;21(1):65-71 PMID: 9741111
  4. Presence of adherent Escherichia coli strains in ileal mucosa of patients with Crohn's disease.
    Gastroenterology. 1998 Dec;115(6):1405-13 PMID: 9834268
  5. Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization.
    Appl Environ Microbiol. 2000 May;66(5):2263-6 PMID: 10788414
  6. Studies of intestinal microflora. II. Microorganisms of the small intestine and their relations to oral and fecal flora.
    Gastroenterology. 1967 Dec;53(6):856-67 PMID: 4863722
  7. The microflora of the human ileum and intrabdominal colon: results of direct needle aspiration at surgery and evaluation of the technique.
    J Lab Clin Med. 1972 Mar;79(3):421-9 PMID: 4551448
  8. Intestinal flora in health and disease.
    Gastroenterology. 1984 Jan;86(1):174-93 PMID: 6357937
  9. Colonic metabolism of wheat starch in healthy humans. Effects on fecal outputs and clinical symptoms.
    Gastroenterology. 1986 Jan;90(1):111-9 PMID: 2998917
  10. Measurement of gastrointestinal pH profiles in normal ambulant human subjects.
    Gut. 1988 Aug;29(8):1035-41 PMID: 3410329
  11. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology.
    J Bacteriol. 1990 Feb;172(2):762-70 PMID: 1688842
  12. The control and consequences of bacterial fermentation in the human colon.
    J Appl Bacteriol. 1991 Jun;70(6):443-59 PMID: 1938669
  13. Detection of bifidobacteria by using propionic acid as a selective agent.
    Appl Environ Microbiol. 1991 Aug;57(8):2418-9 PMID: 1768114
  14. Comparison of fermentation reactions in different regions of the human colon.
    J Appl Bacteriol. 1992 Jan;72(1):57-64 PMID: 1541601
  15. Interrelations between populations of methanogenic archaea and sulfate-reducing bacteria in the human colon.
    FEMS Microbiol Lett. 1992 Nov 1;77(1-3):225-8 PMID: 1459413
  16. Pyxigraphic sampling to enumerate methanogens and anaerobes in the right colon of healthy humans.
    Gastroenterology. 1993 Nov;105(5):1281-5 PMID: 8224632
  17. Spatial distribution of Escherichia coli in the mouse large intestine inferred from rRNA in situ hybridization.
    Infect Immun. 1994 Nov;62(11):5191-4 PMID: 7927805
  18. Digestibility and bulking effect of ispaghula husks in healthy humans.
    Gut. 1994 Dec;35(12):1747-52 PMID: 7829013
  19. Motor activity recorded in the unprepared colon of healthy humans.
    Gut. 1995 Nov;37(5):649-53 PMID: 8549940
  20. Selective enumeration of Bacteroides vulgatus and B. distasonis organisms in the predominant human fecal flora by using monoclonal antibodies.
    Appl Environ Microbiol. 1996 Feb;62(2):735-8 PMID: 8593077
  21. The oligonucleotide probe database.
    Appl Environ Microbiol. 1996 Oct;62(10):3557-9 PMID: 8837410
  22. Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes.
    Appl Environ Microbiol. 1998 Sep;64(9):3336-45 PMID: 9726880
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.67.10.4939-4942.2001
Email
[email protected]
Published
2001-10-00
Pages
4939-42
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC93255
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.67.10.4939-4942.2001 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2