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PMID: 115842 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Restriction enzyme analysis of Bacillus subtilis ribosomal ribonucleic acid genes.

Journal of bacteriology ·Vol. 140 ·No. 1 ·1979-10-00 ·Pages 99-105

Moran CP, Bott KF

Abstract

The organization of the ribosomal ribonucleic acid (rRNA) genes (rDNA) of Bacillus subtilis was examined by cleaving the genome with several restriction endonucleases. The rDNA sequences were assayed by hybridization with purified radioactive rRNA's. Our interpretation of the resulting electrophoretic patterns is strengthened by an analysis of a fragment of B. subtilis rDNA cloned in Escherichia coli. The results indicated that there are eight rRNA operons in B. subtilis. Each operon contains one copy of the sequences coding for 16S, 23S, and 5S rRNA. The sequences coding for 5S rRNA were shown to be more closely linked to the 23S rRNA genes than to the 16S rRNA genes.

MeSH Terms
Bacillus subtilis/genetics Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/analysis,genetics DNA, Recombinant Genes RNA, Bacterial/genetics RNA, Ribosomal/genetics
Chemicals
DNA, Bacterial DNA, Recombinant RNA, Bacterial RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Moran C P
Bott K F
References (17)
17 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.140.1.99-105.1979
Published
1979-10-00
Pages
99-105
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216784
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.140.1.99-105.1979 PMC Full Text View journal details
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