Recombineering: a powerful new tool for mouse functional genomics.

Nature reviews. Genetics ·Vol. 2 ·No. 10 ·2001-10-00 ·Pages 769-79

Copeland NG, Jenkins NA, Court DL

Abstract

Highly efficient phage-based Escherichia coli homologous recombination systems have recently been developed that enable genomic DNA in bacterial artificial chromosomes to be modified and subcloned, without the need for restriction enzymes or DNA ligases. This new form of chromosome engineering, termed recombinogenic engineering or recombineering, is efficient and greatly decreases the time it takes to create transgenic mouse models by traditional means. Recombineering also facilitates many kinds of genomic experiment that have otherwise been difficult to carry out, and should enhance functional genomic studies by providing better mouse models and a more refined genetic analysis of the mouse genome.

MeSH Terms

Animals Bacterial Proteins/physiology Bacteriophage P1/genetics Bacteriophage lambda/genetics Chromosomes, Artificial, Bacterial/genetics Chromosomes, Artificial, P1 Bacteriophage/genetics Chromosomes, Artificial, Yeast/genetics Cloning, Molecular/methods DNA Repair DNA, Bacterial/genetics DNA, Fungal/genetics DNA, Recombinant/genetics DNA, Single-Stranded/genetics DNA-Binding Proteins Escherichia coli/genetics Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/physiology Forecasting Gene Expression Regulation, Viral Genetic Engineering/methods Genomics/methods Mice/genetics Mice, Knockout Mice, Transgenic Rec A Recombinases/metabolism Recombination, Genetic Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Transgenes Viral Proteins/physiology

Chemicals

Bacterial Proteins DNA, Bacterial DNA, Fungal DNA, Recombinant DNA, Single-Stranded DNA-Binding Proteins Escherichia coli Proteins RecT protein, E coli Viral Proteins Rec A Recombinases Exodeoxyribonucleases recE protein, E coli Exodeoxyribonuclease V

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Copeland N G

Jenkins N A

Court D L

Article Info

Journal

Nature reviews. Genetics

Abbr.

Nat Rev Genet

ISSN

1471-0056

DOI

10.1038/35093556

Published

2001-10-00

Pages

769-79

Language

English

Region

England

NLM ID

100962779

Subset

External Links

PubMed source DOI: 10.1038/35093556 View journal details

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Recombineering: a powerful new tool for mouse functional genomics.

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