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PMID: 11598013 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins.

The EMBO journal ·Vol. 20 ·No. 20 ·2001-10-15 ·Pages 5703-14

Walther TC, Fornerod M, Pickersgill H, Goldberg M, Allen TD, Mattaj IW

Abstract

The nuclear pore complex (NPC) is a large proteinaceous structure through which bidirectional transport of macromolecules across the nuclear envelope (NE) takes place. Nup153 is a peripheral NPC component that has been implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Here, Nup153 is localized by immunogold electron microscopy to a position on the nuclear ring of the NPC. Nuclear reconstitution is used to investigate the role of Nup153 in nucleo- cytoplasmic transport and NPC architecture. NPCs assembled in the absence of Nup153 lacked several nuclear basket components, were unevenly distributed in the NE and, unlike wild-type NPCs, were mobile within the NE. Importin alpha/beta-mediated protein import into the nucleus was strongly reduced in the absence of Nup153, while transportin-mediated import was unaffected. This was due to a reduction in import complex translocation rather than to defective receptor recycling. Our results therefore reveal functions for Nup153 in NPC assembly, in anchoring NPCs within the NE and in mediating specific nuclear import events.

MeSH Terms
Animals Cattle Female Heterogeneous-Nuclear Ribonucleoproteins Humans Immunohistochemistry Karyopherins/metabolism Macromolecular Substances Male Microscopy, Immunoelectron Nuclear Pore/physiology,ultrastructure Nuclear Pore Complex Proteins/physiology Nuclear Proteins/genetics,metabolism Nucleoplasmins Oocytes Phosphoproteins/genetics,metabolism Protein Transport/physiology Recombinant Fusion Proteins/metabolism Ribonucleoproteins/genetics,metabolism Serum Albumin, Bovine/genetics,metabolism Xenopus laevis
Chemicals
Heterogeneous-Nuclear Ribonucleoproteins Karyopherins Macromolecular Substances NUP153 protein, human Nuclear Pore Complex Proteins Nuclear Proteins Nucleoplasmins Phosphoproteins Recombinant Fusion Proteins Ribonucleoproteins Serum Albumin, Bovine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Walther T C
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Fornerod M
Pickersgill H
Goldberg M
Allen T D
Mattaj I W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.20.5703
Published
2001-10-15
Pages
5703-14
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125666
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.20.5703 PMC Full Text View journal details
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