Restriction cleavage map of SP01 DNA: general location of early, middle, and late genes.
A detailed restriction endonuclease map for the genome of Bacillus subtilis phage SP01 is presented. Sites of cleavage for the restriction enzymes BglII, EcoRI, HaeIII, and SalI were determined. This physical map showed that SP01 DNA was 140 kilobases in length and contained a repeated sequence of 12.4 kilobases at its termini. Combined with previously published information, we were also able to identify the general locations of genes expressed at early, middle, or late times in the phage lytic cycle. In particular, early genes were largely clustered in the terminal repeats, whereas a major cluster of late genes was located in the left-central portion of the genome.
19 references, click to expand
-
Bacillus subtilis bacteriophages SP82, SPO1, and phie: a comparison of DNAs and of peptides synthesized during infection.J Virol. 1978 Sep;27(3):725-37 PMID: 100607
-
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
-
Detection of specific sequences among DNA fragments separated by gel electrophoresis.J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
-
THE POSSIBLE ROLE OF RECOMBINATION IN THE INFECTION OF COMPETENT BACILLUS SUBTILIS BY BACTERIOPHAGE DEOXYRIBONUCLEIC ACID.Virology. 1964 Dec;24:552-62 PMID: 14240402
-
Specificity of substrate recognition by the EcoRI restriction endonuclease.Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310-4 PMID: 242001
-
Purification of a positive regulatory subunit from phage SP01-modified RNA polymerase.Nature. 1977 Nov 3;270(5632):28-32 PMID: 412108
-
Restriction fragment analysis of the temporal program of bacteriophage SPO1 transcription and its control by phage-modified RNA polymerases.Virology. 1977 Dec;83(2):365-79 PMID: 412317
-
Promoter recognition by phage SP01-modified RNA polymerase.Proc Natl Acad Sci U S A. 1978 Mar;75(3):1185-9 PMID: 418406
-
The genetics of bacteriophage SPO1.Biken J. 1972 Jun;15(2):81-97 PMID: 4628367
-
Transcription during bacteriophage SPO1 development: mutations affecting the program of viral transcription.J Mol Biol. 1971 Apr 28;57(2):301-17 PMID: 4996235
-
Susceptibility of non-thymine containing DNA to four bacterial restriction endonucleases.FEBS Lett. 1975 Jul 15;55(1):278-81 PMID: 806480
-
Regulatory gene 28 of bacteriophage SPO1 codes for a phage-induced subunit of RNA polymerase.J Mol Biol. 1976 Mar 5;101(3):427-33 PMID: 815553
-
Identification of phage SP01 proteins coded by regulatory genes 33 and 34.Nature. 1976 Aug 26;262(5571):748-53 PMID: 822347
-
Bacteriophage SP01 regulatory proteins directing late gene transcription in vitro.Nature. 1976 Aug 26;262(5571):753-7 PMID: 822348
-
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
-
Nucleotide sequence of bacteriophage phi X174 DNA.Nature. 1977 Feb 24;265(5596):687-95 PMID: 870828
-
Physical map of the bacteriophage T5 genome based on the cleavage products of the restriction endonucleases SalI, SmaI, BamI, and HpaI.J Virol. 1977 Oct;24(1):249-60 PMID: 904024
-
EcoRI cleavage of DNA from Bacillus subtilis phage SPO1.Virology. 1978 Apr;85(2):601-5 PMID: 96586
-
Terminal redundancy of "high frequency of recombination" markers of Bacillus subtilis phage SPO1.Virology. 1978 May 15;86(2):530-41 PMID: 96587
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
