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PMID: 11602743 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Infection and activation of monocytes by Marburg and Ebola viruses.

Journal of virology ·Vol. 75 ·No. 22 ·2001-11-00 ·Pages 11025-33

Ströher U, West E, Bugany H, Klenk HD, Schnittler HJ, Feldmann H

Abstract

In this study we investigated the effects of Marburg virus and Ebola virus (species Zaire and Reston) infections on freshly isolated suspended monocytes in comparison to adherent macrophages under culture conditions. Our data showed that monocytes are permissive for both filoviruses. As is the case in macrophages, infection resulted in the activation of monocytes which was largely independent of virus replication. The activation was triggered similarly by Marburg and Ebola viruses, species Zaire and Reston, as indicated by the release of the proinflammatory cytokines interleukin-1beta (IL-1beta), tumor necrosis factor alpha, and IL-6 as well as the chemokines IL-8 and gro-alpha. Our data suggest that infected monocytes may play an important role in the spread of filoviruses and in the pathogenesis of filoviral hemorrhagic disease.

MeSH Terms
Animals Chlorocebus aethiops Ebolavirus/physiology Humans Interleukin-6/biosynthesis Interleukin-8/biosynthesis Marburgvirus/physiology Monocytes/physiology,virology Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha/biosynthesis Vero Cells Virus Replication
Chemicals
Interleukin-6 Interleukin-8 Tumor Necrosis Factor-alpha
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ströher U
Institut für Virologie, Philipps-Universität, D-35037 Marburg, Germany.
West E
Bugany H
Klenk H D
Schnittler H J
Feldmann H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.75.22.11025-11033.2001
Published
2001-11-00
Pages
11025-33
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC114683
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.75.22.11025-11033.2001 PMC Full Text View journal details
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