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PMID: 116192 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of RNA secondary structure by photochemical reversal of psoralen crosslinks.

Nucleic acids research ·Vol. 7 ·No. 3 ·1979-10-10 ·Pages 689-703

Rabin D, Crothers DM

Abstract

Aminomethyltrioxsalen (AMT), a psoralen, is known to cause interstrand crosslinks in double stranded nucleic acids. We have demonstrated the photochemical reversal of this reaction, and have used this result to develop a method for identification of specific sequences which are adjacent because of RNA secondary structure formation. E. coli 5S rRNA is used as a model system. We isolated and characterized a product that is derived from the stem region of 5S RNA.

MeSH Terms
Base Composition Base Sequence Escherichia coli/analysis Furocoumarins/radiation effects Nucleic Acid Conformation RNA, Bacterial Ribonuclease T1 Trioxsalen/analogs & derivatives,radiation effects Ultraviolet Rays
Chemicals
Furocoumarins RNA, Bacterial aminomethyltrioxsalen Ribonuclease T1 Trioxsalen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rabin D
Crothers D M
References (29)
29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.3.689
Published
1979-10-10
Pages
689-703
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328048
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.3.689 PMC Full Text View journal details
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