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PMID: 116197 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The nucleotide sequence of human tRNAGly (anticodon GCC).

Nucleic acids research ·Vol. 7 ·No. 4 ·1979-10-25 ·Pages 959-70

Gupta RC, Roe BA, Randerath K

Abstract

The sequence of tRNAGCCGly from human placenta was determined by recently developed postlabeling techniques. The tRNA was digested completely with RNases T1 and A in the presence of alkaline phosphatase, the oligonucleotides were 3'-terminally (3H)-labeled, mapped on PEI-cellulose thin layers, isolated, and sequenced by methods based on base-specific cleavages. Overlaps were obtained by readout sequencing techniques on polyacrylamide gels and PEI-cellulose thin layers. The thin-layer readout technique was used also to locate and identify modified nucleotides. The primary structure was found to exhibit a large degree of homology (94.6%) with silkworm tRNAGCCGly but only 67.6% homology with human tRNACCCGly.

MeSH Terms
Anticodon Base Sequence Female Glycine Humans Oligoribonucleotides/analysis Placenta/analysis Pregnancy RNA, Transfer/isolation & purification Ribonuclease T1 Ribonucleases
Chemicals
Anticodon Oligoribonucleotides RNA, Transfer Ribonucleases Ribonuclease T1 Glycine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gupta R C
Roe B A
Randerath K
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.4.959
Published
1979-10-25
Pages
959-70
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342274
Subset
IM
Databases
GENBANK
K00208
External Links
PubMed source DOI: 10.1093/nar/7.4.959 PMC Full Text View journal details
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