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PMID: 1167604 Published · ppublish English Journal Article

Ribonucleoproteins of Uukuniemi virus are circular.

Journal of virology ·Vol. 15 ·No. 2 ·1975-02-00 ·Pages 386-92

Pettersson RF, von Bonsdorff CH

Abstract

The internal ribonucleoprotein (RNP) of Uukuniemi virus was released with Triton X-100 and analyzed on sucrose gradients. Three species of RNP sedimenting at 140 to 150, 105 to 120, and 85 to 90S could be separated. All of them contained the same ratio of core polypeptide (mol wt, 25,000) to RNA. Eelctron microscopy using rotatory shadowing showed that all three species were circular. Free ends were rarely seen. Measurements of the strands revealed three distinct length classes of about 2.8, 1.4 and 0.7 mu m. Polyacrylamide-agarose gels showed that the largest RNP contained the L RNA, the medium-sized RNP the M RNA, and the smallest RNP the S RNA.

MeSH Terms
Animals Arboviruses/ultrastructure Cell Line Centrifugation, Density Gradient Chick Embryo Electrophoresis, Polyacrylamide Gel Methionine Microscopy, Electron Nucleoproteins/analysis Phosphates Phosphorus Radioisotopes RNA/analysis Sulfur Radioisotopes Viral Proteins/analysis
Chemicals
Nucleoproteins Phosphates Phosphorus Radioisotopes Sulfur Radioisotopes Viral Proteins RNA Methionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pettersson R F
von Bonsdorff C H
References (24)
24 references, click to expand
  1. ISOLATION OF SOME VIRUSES OTHER THAN TYPICAL TICK-BORNE ENCEPHALITIS VIRUSES FROM IXODES RICINUS TICKS IN FINLAND.
    Ann Med Exp Biol Fenn. 1964;42:109-12 PMID: 14229542
  2. The topology of DNA from the small filamentous bacteriophage fd.
    J Mol Biol. 1966 Jan;15(1):1-7 PMID: 5912041
  3. The structure of the DNA of bacteriophage phi-X174. III. Ultracentrifugal evidence for a ring structure.
    J Mol Biol. 1962 Oct;5:424-34 PMID: 13945085
  4. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
    J Biol Chem. 1961 May;236:1372-9 PMID: 13767412
  5. Molecular weight of poliovirus ribonucleic acid.
    J Virol. 1969 Oct;4(4):475-9 PMID: 4309881
  6. Structure of the ribonucleoprotein of influenza virus.
    J Virol. 1972 Oct;10(4):795-800 PMID: 4117350
  7. The structure of influenza virus. II. A model based on the morphology and composition of subviral particles.
    Virology. 1972 Jan;47(1):181-96 PMID: 4110126
  8. Structural components of Uukuniemi virus, a noncubical tick-borne arbovirus.
    Virology. 1971 Dec;46(3):721-9 PMID: 4109526
  9. The structure of Uukuniemi virus.
    Acta Virol. 1970 Mar;14(2):103-7 PMID: 4192444
  10. The fine structure of vesicular stomatitis virus.
    Virology. 1968 Jun;35(2):268-81 PMID: 4298711
  11. Molecular weight determination of Sendai and Newcastle disease virus RNA.
    J Virol. 1974 Feb;13(2):261-8 PMID: 4359293
  12. Ribonucleic acid species of intracellular nucleocapsids and released virions of vesicular stomatitis virus.
    J Virol. 1972 Aug;10(2):244-55 PMID: 4345058
  13. The phospholipid composition of Uukuniemi virus, a non-cubical tick-borne arbovirus.
    Virology. 1972 Dec;50(3):899-901 PMID: 4344945
  14. Studies on the possibility of obtaining leukosis-free eggs from commercial poultry flocks, with reference to current laboratory methods.
    Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(4):473-8 PMID: 4320953
  15. Passerine birds in the ecology of Uukuniemi virus.
    Med Biol. 1974 Apr;52(2):98-103 PMID: 4837432
  16. The ribonucleic acids of Uukuniemi virus, a noncubical tick-borne arbovirus.
    Virology. 1973 Dec;56(2):608-19 PMID: 4796549
  17. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  18. Electron microscopy of the Uukuniemi virus, an ungrouped arbovirus.
    Virology. 1968 Apr;34(4):804-6 PMID: 4871955
  19. Antigenic characterization of tick-borne arboviruses not belonging to group B.
    Jpn J Med Sci Biol. 1967 Dec;20 Suppl:119-29 PMID: 5301557
  20. Properties of Semliki Forest virus nucleocapsid. 1. Sensitivity to pancreatic ribonuclease.
    Virology. 1971 Jan;43(1):291-9 PMID: 5543280
  21. Studies in subviral components of Semliki Forest virus.
    Ann Med Exp Biol Fenn. 1969;47(4):235-48 PMID: 5393678
  22. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  23. The inner structure of Uukuniemi and two Bunyamwera supergroup arboviruses.
    Virology. 1969 Oct;39(2):342-4 PMID: 5344294
  24. Sedimentation coefficients of encephalomyocarditis virus and its ribonucleic acid.
    Nature. 1963 Mar 23;197:1177-8 PMID: 14017133
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.2.386-392.1975
Published
1975-02-00
Pages
386-92
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354464
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.15.2.386-392.1975 PMC Full Text View journal details
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