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PMID: 11689437 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation.

The EMBO journal ·Vol. 20 ·No. 21 ·2001-11-01 ·Pages 5971-81

Suzuki K, Kirisako T, Kamada Y, Mizushima N, Noda T, Ohsumi Y

Abstract

Macroautophagy is a bulk degradation process induced by starvation in eukaryotic cells. In yeast, 15 Apg proteins coordinate the formation of autophagosomes. Several key reactions performed by these proteins have been described, but a comprehensive understanding of the overall network is still lacking. Based on Apg protein localization, we have identified a novel structure that functions in autophagosome formation. This pre-autophagosomal structure, containing at least five Apg proteins, i.e. Apg1p, Apg2p, Apg5p, Aut7p/Apg8p and Apg16p, is localized in the vicinity of the vacuole. Analysis of apg mutants revealed that the formation of both a phosphatidylethanolamine-conjugated Aut7p and an Apg12p- Apg5p conjugate is essential for the localization of Aut7p to the pre-autophagosomal structure. Vps30p/Apg6p and Apg14p, components of an autophagy- specific phosphatidylinositol 3-kinase complex, Apg9p and Apg16p are all required for the localization of Apg5p and Aut7p to the structure. The Apg1p protein kinase complex functions in the late stage of autophagosome formation. Here, we present the classification of Apg proteins into three groups that reflect each step of autophagosome formation.

MeSH Terms
Autophagy/physiology Autophagy-Related Protein 5 Autophagy-Related Protein 8 Family Autophagy-Related Proteins Carrier Proteins/genetics,metabolism Fungal Proteins/genetics,metabolism Green Fluorescent Proteins Heat-Shock Proteins/genetics,metabolism Luminescent Proteins/genetics Macromolecular Substances Membrane Proteins/genetics,metabolism Microtubule-Associated Proteins/metabolism Mutagenesis Protein Processing, Post-Translational/physiology Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins/genetics,metabolism Temperature Transcription Factors Ubiquitin-Protein Ligases Vacuoles/metabolism Vesicular Transport Proteins
Chemicals
ATG16 protein, S cerevisiae ATG5 protein, S cerevisiae ATG8 protein, S cerevisiae Autophagy-Related Protein 5 Autophagy-Related Protein 8 Family Autophagy-Related Proteins Carrier Proteins Fungal Proteins Heat-Shock Proteins Luminescent Proteins Macromolecular Substances Membrane Proteins Microtubule-Associated Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors VPS30 protein, S cerevisiae Vesicular Transport Proteins Green Fluorescent Proteins Ubiquitin-Protein Ligases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Suzuki K
Department of Cell Biology, National Institute for Basic Biology, Nishigonaka 38, Myodaiji-cho, Okazaki 444-8585, Japan.
Kirisako T
Kamada Y
Mizushima N
Noda T
Ohsumi Y
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.21.5971
Published
2001-11-01
Pages
5971-81
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125692
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.21.5971 PMC Full Text View journal details
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