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PMID: 1170172 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Inhibition by colchicine of the mitogenic stimulation of lymphocytes prior to the S phase.

The Journal of cell biology ·Vol. 66 ·No. 1 ·1975-07-00 ·Pages 128-44

Wang JL, Gunther GR, Edelman GM

Abstract

Colchicine, vinblastine, and vincristine inhibit the mitogenic stimulation of lymphocytes by concanavalin A as measured by the incorporation of [3H]thymidine and the appearance of blast cells. The inhibitory effect of colchicine could not be accounted for by diminution in cell viability or by metaphase arrest of mitosis in the stimulated cells. Moreover, the inhibition of [3H]thymidine incorporation was not due to blockage of thymidine transport or inhibition of DNA synthesis inasmuch as addition of colchicine had no effect on cells in the S phase of the cell cycle. The time of inhibition was correlated with the kinetics of cellular commitment to lectin activation and the kinetic data indicated that colchicine blocks stimulation early in the sequence of events following addition of the mitogen. These findings support the hypothesis that cytoplasmic microtubular function plays a role in the commitment of resting cells to undergo mitotic division.

MeSH Terms
Animals Autoradiography Cells, Cultured Colchicine/pharmacology Concanavalin A/pharmacology DNA/biosynthesis Drug Antagonism Humans Kinetics Lymphocyte Activation/drug effects Lymphocytes/drug effects,metabolism Methylmannosides/pharmacology Mice Rabbits Thymidine/metabolism Tritium Vinblastine/pharmacology Vincristine/pharmacology
Chemicals
Methylmannosides Tritium Concanavalin A Vincristine Vinblastine DNA Colchicine Thymidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang J L
Gunther G R
Edelman G M
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40 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.66.1.128
Published
1975-07-00
Pages
128-44
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109517
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.66.1.128 PMC Full Text View journal details
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