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PMID: 1170340 Published · ppublish English Journal Article

Messenger activity of RNA transcribed in vitro by DNA-RNA polymerase associated to vaccinia virus cores.

Journal of virology ·Vol. 15 ·No. 6 ·1975-06-00 ·Pages 1467-74

Jaureguiberry G, Ben-Hamida F, Chapeville F, Beaud G

Abstract

The coding properties of RNA transcribed in vitro by purified vaccinia cores have been investigated using Krebs ascites tumor cells, L cells, and reticulocyte lysates. Six to 10 proteins synthesized in vitro are separated on polyacrylamide gels by electrophoresis in the presence of sodium dodecyl sulfate. Their molecular weights vary from 10,000 to 44,000. The electrophoretic behavior of these proteins is similar to that of early proteins isolated from infected L cells. The tryptic peptide analysis of one of these proteins indicates similarity in amino acid sequences. These results show fidelity of both in vitro transcription and molecular weight above 44,000 are synthesized in vitro does not seem due to a competition between 12S mRNA synthesized in excess and RNA of a higher sedimentation coefficient present in a lower amount.

MeSH Terms
Animals Carcinoma Carcinoma, Krebs 2 Cell Line Cell-Free System DNA-Directed RNA Polymerases/metabolism Electrophoresis, Polyacrylamide Gel Humans L Cells Methionine/metabolism Mice Mouth Neoplasms Peptides/analysis Protein Biosynthesis RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Reticulocytes Sulfur Radioisotopes Transcription, Genetic Trypsin Vaccinia virus/analysis,enzymology,metabolism Viral Proteins/analysis,biosynthesis
Chemicals
Peptides RNA, Messenger RNA, Viral Sulfur Radioisotopes Viral Proteins Methionine DNA-Directed RNA Polymerases Trypsin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jaureguiberry G
Ben-Hamida F
Chapeville F
Beaud G
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31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.6.1467-1474.1975
Published
1975-06-00
Pages
1467-74
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354614
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.15.6.1467-1474.1975 PMC Full Text View journal details
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