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PMID: 11711432 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Amphiphysin is necessary for organization of the excitation-contraction coupling machinery of muscles, but not for synaptic vesicle endocytosis in Drosophila.

Genes & development ·Vol. 15 ·No. 22 ·2001-11-15 ·Pages 2967-79

Razzaq A, Robinson IM, McMahon HT, Skepper JN, Su Y, Zelhof AC, Jackson AP, Gay NJ, O'Kane CJ

Abstract

Amphiphysins 1 and 2 are enriched in the mammalian brain and are proposed to recruit dynamin to sites of endocytosis. Shorter amphiphysin 2 splice variants are also found ubiquitously, with an enrichment in skeletal muscle. At the Drosophila larval neuromuscular junction, amphiphysin is localized postsynaptically and amphiphysin mutants have no major defects in neurotransmission; they are also viable, but flightless. Like mammalian amphiphysin 2 in muscles, Drosophila amphiphysin does not bind clathrin, but can tubulate lipids and is localized on T-tubules. Amphiphysin mutants have a novel phenotype, a severely disorganized T-tubule/sarcoplasmic reticulum system. We therefore propose that muscle amphiphysin is not involved in clathrin-mediated endocytosis, but in the structural organization of the membrane-bound compartments of the excitation-contraction coupling machinery of muscles.

MeSH Terms
Animals Brain/metabolism Calcium/pharmacology Clathrin/metabolism DNA, Complementary/metabolism Drosophila/metabolism Electrophysiology Endocytosis Glutathione Transferase/metabolism Immunohistochemistry Microscopy, Confocal Microscopy, Fluorescence Models, Genetic Muscle, Skeletal/metabolism Muscles/metabolism Mutation Nerve Tissue Proteins/metabolism,physiology Neuromuscular Junction Phenotype Protein Binding Protein Structure, Tertiary Rats Recombinant Fusion Proteins/metabolism Sarcoplasmic Reticulum Tissue Distribution Tubulin/metabolism
Chemicals
Clathrin DNA, Complementary Nerve Tissue Proteins Recombinant Fusion Proteins Tubulin amphiphysin Glutathione Transferase Calcium
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Razzaq A
Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.
Robinson I M
McMahon H T
Skepper J N
Su Y
Zelhof A C
Jackson A P
Gay N J
O'Kane C J
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.207801
Published
2001-11-15
Pages
2967-79
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312829
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.207801 PMC Full Text View journal details
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