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PMID: 1172612 Published · ppublish English Journal Article

Contribution of an electrogenic sodium pump to the membrane potential in rabbit sinoatrial node cells.

Pflugers Archiv : European journal of physiology ·Vol. 358 ·No. 4 ·1975-08-12 ·Pages 289-301

Noma A, Irisawa H

Abstract

A study has been made of the transient hyperpolarization (K+-induced hyperpolarization) which developed following readmission of potassium after having pre-treated the rabbit sinoatrial node tissue with K+-depleted Tyrode solution for 4--5 min at 35 degrees C. Evidence is presented indicating that the K+-induced hyperpolarization results from the activity of an electrogenic sodium pump: The K+-induced hyperpolarization was inhibited by substituting Li+ for Na+ and by cooling the tissue. The amplitude of the K+-induced hyperpolarization was increased either by increasing K+ concentration in the recovery solution or by decreasing K+ concentration in the pre-treatment K+-depleted solution. By removing Cl- from the perfusates, the amplitude of the K+-induced hyperpolarization increased. In a Cl--depleted solution, the sinoatrial node cell membrane hyperpolarized by approximately 15 mV without a transient depolarization.

MeSH Terms
Animals Biological Transport, Active Cell Membrane Permeability Chlorides/pharmacology Lithium Membrane Potentials Potassium Rabbits/physiology Sinoatrial Node/physiology Sodium/metabolism Solutions
Chemicals
Chlorides Solutions Lithium Sodium Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Noma A
Irisawa H
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31 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00580527
Published
1975-08-12
Pages
289-301
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00580527 View journal details
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