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PMID: 11739803 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Lipid composition of outer leaflet of chloroplast outer envelope determines topology of OEP7.

Molecular biology of the cell ·Vol. 12 ·No. 12 ·2001-12-00 ·Pages 4090-102

Schleiff E, Tien R, Salomon M, Soll J

Abstract

OEP7, a 6.7-kDa outer envelope protein of spinach chloroplasts inserts into the outer envelope of the organelle independent of a classical cleavable targeting signal. The insertion of OEP7 was studied to describe the determinants for association with, integration into, and orientation of the protein in the outer envelope of chloroplasts. The insertion of OEP7 into the membrane is independent of outer membrane channel proteins and can be reconstituted with the use of protein-free liposomes. In situ, the binding of OEP7 to the membrane surface is not driven by electrostatic interaction because reduction of phosphatidylglycerol or phosphatidylinositol did not reduce the association with the liposomes. The positively charged amino acids flanking the transmembrane domain at the C terminus are essential to retain the native N(in)-C(out) orientation during insertion into chloroplasts. OEP7 inserts with reversed orientation into liposomes containing the average lipid composition of the outer envelopes. The native like N(in)-C(out) orientation is achieved by reduction of the phoshpatidylglycerol concentration mimicking the composition of the outer leaflet of the outer envelope of chloroplasts. We conclude that the unique lipid composition of the outer leaflet due to lipid asymmetry of the outer envelope is essential for the correct topology of OEP7.

MeSH Terms
Chloroplasts/chemistry Hydrophobic and Hydrophilic Interactions Liposomes/chemistry,metabolism Membrane Lipids/analysis,metabolism Membrane Proteins/chemistry,genetics,metabolism Models, Biological Phosphatidylglycerols/metabolism Phosphatidylinositols/metabolism Plant Proteins/chemistry,genetics,metabolism Protein Conformation Spinacia oleracea/genetics Static Electricity Thermodynamics
Chemicals
Liposomes Membrane Lipids Membrane Proteins Phosphatidylglycerols Phosphatidylinositols Plant Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schleiff E
Botanisches Institut, Universität Kiel, 24118 Kiel, Germany.
Tien R
Salomon M
Soll J
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.12.12.4090
Published
2001-12-00
Pages
4090-102
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC60778
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.12.12.4090 PMC Full Text View journal details
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