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PMID: 1176430 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transformation analysis of three linkage groups in Staphylococcus aureus.

Journal of bacteriology ·Vol. 124 ·No. 1 ·1975-10-00 ·Pages 201-11

Pattee PA, Neveln DS

Abstract

While studying a set of multiply marked mutants of Staphylococcus aureus strain 8325 by transformation, several instances of apparent genetic linkage were encountered. After showing that these linked transformations were readily inactivated by shearing of the deoxyribonucleic acid (DNA) but were resistant to dilution of the DNA, and showing that mixtures of DNA failed to form double transformants, it was concluded that the linkages were legitimate rather than the result of congression. Three linkage groups were defined: thy-101-lys-115-trp-103-thr-106, pyr-141-hisGb15-nov-pur-102, and pur-110-ilv-129. The positions of the previously studied trp and his operons corresponded to the trp-103 and hisGb15 loci. The ilv-129 position adjacent to pur-110 probably corresponds to the ilv-leu gene cluster. The distance over which linkage was detected was greater by transformation than by generalized transduction.

MeSH Terms
Amino Acids/biosynthesis Chromosome Mapping DNA, Bacterial Genes Genetic Linkage Mutation Purines/biosynthesis Pyrimidines/biosynthesis Staphylococcus aureus/metabolism Transformation, Genetic
Chemicals
Amino Acids DNA, Bacterial Purines Pyrimidines
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pattee P A
Neveln D S
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.124.1.201-211.1975
Published
1975-10-00
Pages
201-11
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235883
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.124.1.201-211.1975 PMC Full Text View journal details
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