Rapid method to detect rubella immunoglobulin M and immunoglobulin A antibodies.

Journal of clinical microbiology ·Vol. 1 ·No. 2 ·1975-02-00 ·Pages 132-5

Schmitz H, Shimizu H, Kampa D, Doerr HW

Abstract

Immunoglobulin (Ig) G was removed from serum specimens by precipitation with gamma chain-specific anti-human IgG of rabbit origin. The remaining rubella virus-specific IgM (and IgA) antibodies were then detected by the rubella hemagglutination-inhibition test. This procedure has proven to be as reliable as estimations carried out with IgM fractions separated on a sucrose density gradient.

MeSH Terms

Adult Antibodies, Viral/analysis Chemical Precipitation Evaluation Studies as Topic Hemagglutination Inhibition Tests Humans Immunoglobulin A/analysis Immunoglobulin G/analysis,isolation & purification Immunoglobulin M/analysis Rubella/immunology Rubella virus/immunology

Chemicals

Antibodies, Viral Immunoglobulin A Immunoglobulin G Immunoglobulin M

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Schmitz H

Shimizu H

Kampa D

Doerr H W

References (8)

8 references, click to expand

Separation of rubella IgM, IgA, and IgG antibodies by gel filtration on agarose.
Lancet. 1971 Dec 11;2(7737):1278-80 PMID: 4143534
IgM antibodies to Epstein-Barr virus in infectious mononucleosis.
Arch Gesamte Virusforsch. 1972;37(4):332-9 PMID: 4339106
Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies.
Br Med J. 1968 Jan 27;1(5586):221-3 PMID: 4169330
Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides.
Nature. 1967 Jun 24;214(5095):1302-4 PMID: 6056841
Demonstration of rubella IgM antibody by indirect fluorescent antibody staining, sucrose density gradient centrifugation and mercaptoethanol reduction.
Intervirology. 1973;1(1):48-59 PMID: 4204098
IgG, IgA and IgM responses in acute rubella determined by the immunofluorescent technique.
J Hyg (Lond). 1972 Sep;70(3):473-85 PMID: 4627265
Immunochemical quantitation of antigens by single radial immunodiffusion.
Immunochemistry. 1965 Sep;2(3):235-54 PMID: 4956917
Determination of different cytomegalovirus immunoglobulins (IgG, IgA, IgM) by immunofluorescence.
Arch Gesamte Virusforsch. 1972;37(1):131-40 PMID: 4336960

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.1.2.132-135.1975

Published

1975-02-00

Pages

132-5

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC274987

Subset

External Links

PubMed source DOI: 10.1128/jcm.1.2.132-135.1975 PMC Full Text View journal details

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