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PMID: 11772648 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phylogeny of culturable estuarine bacteria catabolizing riverine organic matter in the northern Baltic Sea.

Applied and environmental microbiology ·Vol. 68 ·No. 1 ·2002-01-00 ·Pages 379-88

Kisand V, Cuadros R, Wikner J

Abstract

The objective of our study was to isolate and determine the phylogenetic affiliation of culturable estuarine bacteria capable of catabolizing riverine dissolved organic matter (RDOM) under laboratory conditions. Additions of RDOM consistently promoted the growth of estuarine bacteria in carbon-limited dilution cultures, with seasonal variation in growth rates and yields. At least 42 different taxa were culturable on solid agar media and, according to quantitative DNA-DNA hybridizations, constituted 32 to 89% of the total bacterial number in the enriched treatments. Five species in the Cytophaga-Flexibacter-Bacteroides group and one in the gamma-proteobacteria phylogenetic group (Marinomonas sp.) were numerically dominant during the stationary phase of the RDOM-enriched dilution cultures but not in the control cultures. Four of the isolates in Cytophaga-Flexibacter-Bacteroides group were putatively affiliated with the genus FLAVOBACTERIUM: All dominating isolates were determined to be new species based on comparison to the current databases. The same group of species dominated independently of the season investigated, suggesting a low diversity of bacteria catabolizing RDOM in the estuary. It also suggested a broad tolerance of the dominating species to seasonal variation in hydrography, chemistry, and competition with other species. Taken together, our results suggest that a limited group of bacteria, mainly in the Flavobacterium genus, played an important role in introducing new energy and carbon to the marine system in the northern Baltic Sea.

MeSH Terms
Bacteria/classification,genetics,growth & development,isolation & purification Bacteroides/classification,genetics,growth & development,isolation & purification Baltic States Colony Count, Microbial Culture Media Cytophaga/classification,genetics,growth & development,isolation & purification Fresh Water/chemistry,microbiology Gammaproteobacteria/genetics,growth & development,isolation & purification Molecular Sequence Data Organic Chemicals/metabolism Phylogeny RNA, Ribosomal, 16S/genetics Seasons Sequence Analysis, DNA
Chemicals
Culture Media Organic Chemicals RNA, Ribosomal, 16S
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kisand Veljo
Department of Microbiology, Umeå University, S-901 87, Umeå, Sweden.
Cuadros Rocio
Wikner Johan
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.68.1.379-388.2002
Published
2002-01-00
Pages
379-88
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC126561
Subset
IM
Databases
GENBANK
AF321008, AF321009, AF321010, AF321011, AF321012, AF321013, AF321014, AF321015, AF321016, AF321017, AF321018, AF321019, AF321020, AF321021, AF321022, AF321023, AF321024, AF321025, AF321026, AF321027, AF321028, AF321029, AF321030, AF321031, AF321032, AF321033, AF321034, AF321035, AF321036, AF321037, AF321038, AF321039, AF321040, AF321041, AF321042, AF321043, AF321044, AF321045, AF321046, AF321047, AF321048, AF321049
External Links
PubMed source DOI: 10.1128/AEM.68.1.379-388.2002 PMC Full Text View journal details
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