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PMID: 11773119 Published · ppublish English Evaluation Study Journal Article

Serotype identification of group B streptococci by PCR and sequencing.

Journal of clinical microbiology ·Vol. 40 ·No. 1 ·2002-01-00 ·Pages 216-26

Kong F, Gowan S, Martin D, James G, Gilbert GL

Abstract

Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Ongoing surveillance to monitor GBS serotype distribution will be needed to guide the development and use of GBS conjugate vaccines. We designed sequencing primers based on the previously published sequences of the capsular polysaccharide (cps) gene clusters to further define partial cps gene clusters for eight of the nine GBS serotypes (serotypes Ia to VII). Subsequently, we designed and evaluated primers to identify serotypes Ia, Ib, III, IV, V, and VI directly by PCR and all eight serotypes (serotypes Ia to VII) by sequence heterogeneity. A total of 206 clinical GBS isolates were used to compare our molecular serotype (MS) identification method with conventional serotyping (CS). All clinical isolates were assigned an MS, whereas 188 of 206 (91.3%) were assigned a serotype by use of antisera. A small number of isolates (serosubtypes III-3 and III-4) showed different serotype specificities between PCR and sequencing, but the PCR results correlated with those obtained by CS. The overall agreement between the MS identification method and CS for isolates for which results of both tests were available was 100% (188 of 188 isolates). The MS identification method is a specific and practical alternative to conventional GBS serotyping and will facilitate epidemiological studies.

MeSH Terms
Bacterial Capsules/genetics Base Sequence Genetic Variation Humans Molecular Sequence Data Polymerase Chain Reaction/methods Sequence Analysis, DNA Serotyping Streptococcal Infections/microbiology Streptococcus agalactiae/classification,genetics
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kong Fanrong
Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia.
Gowan Sonia
Martin Diana
James Gregory
Gilbert Gwendolyn L
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.40.1.216-226.2002
Published
2002-01-00
Pages
216-26
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC120111
Subset
IM
Databases
GENBANK
AF291411, AF291412, AF291413, AF291414, AF291415, AF291416, AF291417, AF291418, AF291419, AF332893, AF332894, AF332895, AF332896, AF332897, AF332898, AF332899, AF332900, AF332901, AF332902, AF332903, AF332904, AF332905, AF332906, AF332907, AF332908, AF332909, AF332910, AF332911, AF332912, AF332913, AF332914, AF332915, AF332916, AF332917, AF363032, AF363033, AF363034, AF363035, AF363036, AF363037, AF363038, AF363039, AF363040, AF363041, AF363042, AF363043, AF363044, AF363045, AF363046, AF363047, AF363048, AF363049, AF363050, AF363051, AF363052, AF363053, AF363054, AF363055, AF363056, AF363057, AF363058, AF363059, AF363060, AF367973, AF381030, AF381031
External Links
PubMed source DOI: 10.1128/JCM.40.1.216-226.2002 PMC Full Text View journal details
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