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PMID: 11778012 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning.

Science (New York, N.Y.) ·Vol. 295 ·No. 5557 ·2002-02-08 ·Pages 1089-92

Lai L, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, Murphy CN, Carter DB, Hawley RJ, Prather RS

Abstract

The presence of galactose alpha-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the alpha-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.

MeSH Terms
Alleles Animals Animals, Genetically Modified Cell Line Cloning, Organism Embryo Transfer Female Fetus Fibroblasts Galactosyltransferases/genetics Gene Targeting Genetic Vectors Male Mutagenesis, Insertional Nuclear Transfer Techniques Pregnancy Recombination, Genetic Swine Swine, Miniature/embryology,genetics Transfection
Chemicals
Galactosyltransferases N-acetyllactosaminide alpha-1,3-galactosyltransferase
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Lai Liangxue
Department of Animal Science, University of Missouri, Columbia, MO 65211, USA.
Kolber-Simonds Donna
Park Kwang-Wook
Cheong Hee-Tae
Greenstein Julia L
Im Gi-Sun
Samuel Melissa
Bonk Aaron
Rieke August
Day Billy N
Murphy Clifton N
Carter David B
Hawley Robert J
Prather Randall S
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1068228
Published
2002-02-08
Epub
2002-00-03
Pages
1089-92
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Grants
NCRR NIH HHS · R44 RR15198 · United States
NCRR NIH HHS · T32 RR07004 · United States
External Links
PubMed source DOI: 10.1126/science.1068228 View journal details
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