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PMID: 11788750 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional analysis of tomato Pti4 in Arabidopsis.

Plant physiology ·Vol. 128 ·No. 1 ·2002-01-00 ·Pages 30-7

Wu K, Tian L, Hollingworth J, Brown DC, Miki B

Abstract

Pti4 is a tomato (Lycopersicon esculentum) transcription factor that belongs to the ERF (ethylene-responsive element binding factor) family of proteins. It interacts with the Pto kinase in tomato, which confers resistance to the Pseudomonas syringae pv tomato pathogen that causes bacterial speck disease. To study the function of Pti4, transgenic Arabidopsis plants were generated that expressed tomato Pti4 driven by the strong constitutive promoters, cauliflower mosaic virus 35S and tCUP. Global gene expression analysis by Affimetric GeneChip indicated that expression of Pti4 in transgenic Arabidopsis plants induced the expression of GCC box-containing PR genes. We also demonstrated that Pti4 enhanced GCC box-mediated transcription of a reporter gene. The data suggests that tomato Pti4 could act as a transcriptional activator to regulate expression of GCC box-containing genes. Furthermore, we show that the expression of tomato Pti4 in transgenic Arabidopsis plants produced a phenotype similar to that seen in plants treated with ethylene, thus providing evidence that the Pti4 gene is involved in the regulation of a subset of ethylene-responsive genes containing the GCC box.

MeSH Terms
Arabidopsis/drug effects,genetics,growth & development Caulimovirus DNA, Complementary/genetics DNA-Binding Proteins/genetics,physiology Ethylenes/pharmacology Lycopersicon esculentum/genetics Mutation Phenotype Plant Growth Regulators/pharmacology Plant Proteins/genetics,physiology Plants, Genetically Modified Protein Kinases/metabolism Transcription Factors/genetics,physiology
Chemicals
DNA, Complementary DNA-Binding Proteins Ethylenes Plant Growth Regulators Plant Proteins Pti4 protein, Lycopersicon esculentum Transcription Factors ethylene Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wu Keqiang
Department of Biology, West Virginia University, Morgantown, West Virginia 26506-6057, USA.
Tian Lining
Hollingworth Jamie
Brown Daniel C W
Miki Brian
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
Published
2002-01-00
Pages
30-7
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC148941
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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