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PMID: 11818558 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 3 ·2002-02-05 ·Pages 1158-63

Rohdich F, Hecht S, Gärtner K, Adam P, Krieger C, Amslinger S, Arigoni D, Bacher A, Eisenreich W

Abstract

Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-D-xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier in vivo experiments with recombinant Escherichia coli strains showed that exogenous 1-deoxy-D-xylulose can be converted into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate by the consecutive action of enzymes specified by the xylB and ispCDEFG genes. This article describes the transformation of exogenous [U-(13)C(5)]1-deoxy-D-xylulose into a 5:1 mixture of [U-(13)C(5)]isopentenyl diphosphate and [U-(13)C(5)]dimethylallyl diphosphate by an E. coli strain engineered for the expression of the ispH (lytB) gene in addition to recombinant xylB and ispCDEFG genes.

MeSH Terms
Bacterial Proteins/genetics,metabolism Base Sequence Enterobacteriaceae/genetics Escherichia coli/genetics,metabolism Escherichia coli Proteins Magnetic Resonance Spectroscopy Mevalonic Acid/metabolism Molecular Sequence Data Mycobacterium tuberculosis/genetics Oligodeoxyribonucleotides/chemistry Operon Oxidoreductases Promoter Regions, Genetic Recombinant Proteins/metabolism Terpenes/metabolism
Chemicals
Bacterial Proteins Escherichia coli Proteins Oligodeoxyribonucleotides Recombinant Proteins Terpenes Oxidoreductases ispH protein, E coli Mevalonic Acid
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Rohdich Felix
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany. [email protected]
Hecht Stefan
Gärtner Katrin
Adam Petra
Krieger Cornelia
Amslinger Sabine
Arigoni Duilio
Bacher Adelbert
Eisenreich Wolfgang
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.032658999
Email
[email protected]
Published
2002-02-05
Epub
2002-00-29
Pages
1158-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC122160
Subset
IM
Databases
GENBANK
AY062212
External Links
PubMed source DOI: 10.1073/pnas.032658999 PMC Full Text View journal details
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