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PMID: 11826297 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SGR2, a phospholipase-like protein, and ZIG/SGR4, a SNARE, are involved in the shoot gravitropism of Arabidopsis.

The Plant cell ·Vol. 14 ·No. 1 ·2002-01-00 ·Pages 33-46

Kato T, Morita MT, Fukaki H, Yamauchi Y, Uehara M, Niihama M, Tasaka M

Abstract

In higher plants, the shoot and the root generally show negative and positive gravitropism, respectively. To elucidate the molecular mechanisms involved in gravitropism, we have isolated many shoot gravitropism mutants in Arabidopsis. The sgr2 and zig/sgr4 mutants exhibited abnormal gravitropism in both inflorescence stems and hypocotyls. These genes probably are involved in the early step(s) of the gravitropic response. The sgr2 mutants also had misshapen seed and seedlings, whereas the stem of the zig/sgr4 mutants elongated in a zigzag fashion. The SGR2 gene encodes a novel protein that may be part of a gene family represented by bovine phosphatidic acid-preferring phospholipase A1 containing a putative transmembrane domain. This gene family has been reported only in eukaryotes. The ZIG gene was found to encode AtVTI11, a protein that is homologous with yeast VTI1 and is involved in vesicle transport. Our observations suggest that the two genes may be involved in a vacuolar membrane system that affects shoot gravitropism.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,growth & development,radiation effects Arabidopsis Proteins/genetics,metabolism Chromosome Mapping Cloning, Molecular Gene Expression Regulation, Plant/radiation effects Genetic Complementation Test Gravitropism/genetics,physiology,radiation effects Light Membrane Proteins/genetics,metabolism Molecular Sequence Data Mutation Phenotype Phospholipases/genetics,metabolism Plant Proteins/genetics,metabolism Plant Shoots/genetics,growth & development,radiation effects Plants, Genetically Modified Qb-SNARE Proteins Sequence Homology, Amino Acid Signal Transduction Vacuoles/metabolism
Chemicals
Arabidopsis Proteins Membrane Proteins Plant Proteins Qb-SNARE Proteins vesicle transport v-SNARE 11 protein, Arabidopsis vesicle transport v-SNARE 12 protein, Arabidopsis Phospholipases SGR2 phospholipase, Arabidopsis
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kato Takehide
Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Morita Miyo Terao
Fukaki Hidehiro
Yamauchi Yoshiro
Uehara Michiko
Niihama Mitsuru
Tasaka Masao
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010215
Published
2002-01-00
Pages
33-46
Language
English
Region
England
NLM ID
9208688
PMCID
PMC150549
Subset
IM
Databases
GENBANK
AB073133
External Links
PubMed source DOI: 10.1105/tpc.010215 PMC Full Text View journal details
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