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PMID: 11842207 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

2',3'-Cyclic nucleotide 3'-phosphodiesterase: a membrane-bound, microtubule-associated protein and membrane anchor for tubulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 4 ·2002-02-19 ·Pages 1807-12

Bifulco M, Laezza C, Stingo S, Wolff J

Abstract

2',3'-Cyclic nucleotide-3'-phosphodiesterase (CNP) is firmly associated with tubulin from brain tissue and FRTL-5 thyroid cells as demonstrated by copolymerization with microtubules through several warm/cold cycles, the presence of CNP activity in purified tubulin preparations, and identical behavior during various extraction procedures. CNP acts as a microtubule-associated protein in promoting microtubule assembly at low mole ratios. This activity resides in the C terminus of the enzyme, which, by itself, promotes microtubule assembly at higher mole ratios. Phosphorylation of CNP interferes with its assembly-promoting activity, as does deletion of the C terminus, which leads to abnormal microtubule distribution in the cell. Submembranous colocalization of the proteins and CNP-dependent microtubule organization suggest that CNP is a membrane-bound microtubule-associated protein that can link tubulin to membranes and may regulate cytoplasmic microtubule distribution.

MeSH Terms
2',3'-Cyclic-Nucleotide Phosphodiesterases/chemistry,metabolism,physiology Amino Acid Sequence Animals Brain/cytology Cell Line Cell Membrane/metabolism Cytoplasm/chemistry,metabolism DNA, Complementary/metabolism Electrophoresis, Polyacrylamide Gel Genetic Vectors Glutathione Transferase/metabolism Green Fluorescent Proteins Immunoblotting Luminescent Proteins/metabolism Microscopy, Fluorescence Microtubules/chemistry,metabolism Molecular Sequence Data Phosphorylation Protein Binding Protein Structure, Tertiary Rats Recombinant Fusion Proteins/metabolism Subcellular Fractions Thyroid Gland/cytology Time Factors Transfection Tubulin/chemistry,metabolism
Chemicals
DNA, Complementary Luminescent Proteins Recombinant Fusion Proteins Tubulin Green Fluorescent Proteins Glutathione Transferase 2',3'-Cyclic-Nucleotide Phosphodiesterases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bifulco Maurizio
Dipartimento di Scienze Farmaceutiche, Università di Salerno, 84084 Fisciano, Salerno, Italy. [email protected]
Laezza Chiara
Stingo Stefania
Wolff J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.042678799
Email
[email protected]
Published
2002-02-19
Epub
2002-00-12
Pages
1807-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC122275
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.042678799 PMC Full Text View journal details
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