Abstract
Specific combinations of amino acids or purine ribonucleosides and amino acids are required for efficient germination of endospores of Bacillus anthracis DeltaSterne, a plasmidless strain, at ligand concentrations in the low-micromolar range. The amino acid L-alanine was the only independent germinant in B. anthracis and then only at concentrations of >10 mM. Inosine and L-alanine both play major roles as cogerminants with several other amino acids acting as efficient cogerminants (His, Pro, Trp, and Tyr combining with L-alanine and Ala, Cys, His, Met, Phe, Pro, Ser, Trp, Tyr, and Val combining with inosine). An ortholog to the B. subtilis tricistronic germination receptor operon gerA was located on the B. anthracis chromosome and named gerS. Disruption of gerS completely eliminated the ability of B. anthracis endospores to respond to amino-acid and inosine-dependent germination responses. The gerS mutation also produced a significant microlag in the aromatic-amino-acid-enhanced-alanine germination pathways. The gerS disruption appeared to specifically affect use of aromatic chemicals as cogerminants with alanine and inosine. We conclude that efficient germination of B. anthracis endospores requires multipartite signals and that gerS-encoded proteins act as an aromatic-responsive germination receptor.
MeSH Terms
Alanine/pharmacology
Amino Acids/chemistry,pharmacology
Bacillus anthracis/drug effects,genetics,physiology
Bacterial Proteins/genetics,metabolism
Gene Expression Regulation, Bacterial
Hydrogen-Ion Concentration
Inosine/pharmacology
Operon
Plasmids/genetics
Purine Nucleosides/chemistry,pharmacology
Ribonucleosides/chemistry,pharmacology
Spores, Bacterial/drug effects,physiology
Chemicals
Amino Acids
Bacterial Proteins
Purine Nucleosides
Ribonucleosides
Inosine
nebularine
Alanine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ireland John A W
Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Hanna Philip C
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