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PMID: 11854363 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Virus-induced interferon alpha production by a dendritic cell subset in the absence of feedback signaling in vivo.

The Journal of experimental medicine ·Vol. 195 ·No. 4 ·2002-02-18 ·Pages 507-16

Barchet W, Cella M, Odermatt B, Asselin-Paturel C, Colonna M, Kalinke U

Abstract

An effective type I interferon (IFN-alpha/beta) response is critical for the control of many viral infections. Here we show that in vesicular stomatitis virus (VSV)-infected mouse embryonic fibroblasts (MEFs) the production of IFN-alpha is dependent on type I IFN receptor (IFNAR) triggering, whereas in infected mice early IFN-alpha production is IFNAR independent. In VSV-infected mice type I IFN is produced by few cells located in the marginal zone of the spleen. Unlike other dendritic cell (DC) subsets, FACS((R))-sorted CD11c(int)CD11b(-)GR-1(+) DCs show high IFN-alpha expression, irrespective of whether they were isolated from VSV-infected IFNAR-competent or -deficient mice. Thus, VSV preferentially activates a specialized DC subset presumably located in the marginal zone to produce high-level IFN-alpha largely independent of IFNAR feedback signaling.

MeSH Terms
Animals Cells, Cultured DNA-Binding Proteins/metabolism Dendritic Cells/drug effects,metabolism,virology Feedback, Physiological Fibroblasts/metabolism,virology Flow Cytometry Gene Deletion Gene Expression Regulation/drug effects In Situ Hybridization Interferon Regulatory Factor-7 Interferon-alpha/biosynthesis,genetics,metabolism Interferon-beta/metabolism Membrane Proteins Mice Mice, Inbred C57BL Mice, Mutant Strains Poly I-C/pharmacology RNA, Messenger/genetics,metabolism Receptor, Interferon alpha-beta Receptors, Interferon/deficiency,genetics,metabolism Signal Transduction/drug effects Spleen/metabolism,pathology Time Factors Ultraviolet Rays Vesicular stomatitis Indiana virus/physiology
Chemicals
DNA-Binding Proteins Interferon Regulatory Factor-7 Interferon-alpha Irf7 protein, mouse Membrane Proteins RNA, Messenger Receptors, Interferon Receptor, Interferon alpha-beta Interferon-beta Poly I-C
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Barchet Winfried
Mouse Biology Programme, EMBL-Monterotondo, I-00016 Monterotondo-Scalo, Rome, Italy.
Cella Marina
Odermatt Bernhard
Asselin-Paturel Carine
Colonna Marco
Kalinke Ulrich
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.20011666
Published
2002-02-18
Pages
507-16
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2193622
Subset
IM
Corrections
CommentIn
PMID: 11854366
External Links
PubMed source DOI: 10.1084/jem.20011666 PMC Full Text View journal details
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