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PMID: 11861547 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic interactions of yeast eukaryotic translation initiation factor 5A (eIF5A) reveal connections to poly(A)-binding protein and protein kinase C signaling.

Genetics ·Vol. 160 ·No. 2 ·2002-02-00 ·Pages 393-405

Valentini SR, Casolari JM, Oliveira CC, Silver PA, McBride AE

Abstract

The highly conserved eukaryotic translation initiation factor eIF5A has been proposed to have various roles in the cell, from translation to mRNA decay to nuclear protein export. To further our understanding of this essential protein, three temperature-sensitive alleles of the yeast TIF51A gene have been characterized. Two mutant eIF5A proteins contain mutations in a proline residue at the junction between the two eIF5A domains and the third, strongest allele encodes a protein with a single mutation in each domain, both of which are required for the growth defect. The stronger tif51A alleles cause defects in degradation of short-lived mRNAs, supporting a role for this protein in mRNA decay. A multicopy suppressor screen revealed six genes, the overexpression of which allows growth of a tif51A-1 strain at high temperature; these genes include PAB1, PKC1, and PKC1 regulators WSC1, WSC2, and WSC3. Further results suggest that eIF5A may also be involved in ribosomal synthesis and the WSC/PKC1 signaling pathway for cell wall integrity or related processes.

MeSH Terms
Alleles Mutation, Missense Peptide Initiation Factors/genetics,physiology Poly(A)-Binding Proteins Protein Kinase C/genetics,physiology RNA-Binding Proteins/genetics,physiology Saccharomyces cerevisiae/genetics,physiology Signal Transduction/genetics Temperature
Chemicals
Peptide Initiation Factors Poly(A)-Binding Proteins RNA-Binding Proteins eukaryotic translation initiation factor 5A Protein Kinase C
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Valentini Sandro R
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Casolari Jason M
Oliveira Carla C
Silver Pamela A
McBride Anne E
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/160.2.393
Published
2002-02-00
Pages
393-405
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461981
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/160.2.393 PMC Full Text View journal details
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