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PMID: 11861840 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

High-level primary CD8(+) T-cell response to human immunodeficiency virus type 1 gag and env generated by vaccination with recombinant vesicular stomatitis viruses.

Journal of virology ·Vol. 76 ·No. 6 ·2002-03-00 ·Pages 2730-8

Haglund K, Leiner I, Kerksiek K, Buonocore L, Pamer E, Rose JK

Abstract

We investigated the primary cellular immune responses to human immunodeficiency virus type 1 (HIV-1) Env and Gag proteins elicited by recombinant vesicular stomatitis viruses (rVSVs). The primary response to Env peaked 5 to 7 days after intraperitoneal vaccination, at which time 40% of CD8(+) cells were Env tetramer positive and activated (CD62L(Lo)). These freshly isolated cells actively lysed target cells pulsed with the p18-I10 peptide and secreted gamma interferon and tumor necrosis factor alpha after stimulation with the Env p18-I10 peptide. The primary response to Env elicited by rVSVs was sixfold higher than that elicited by recombinant vaccinia viruses (rVVs) at 5 days postvaccination. An intranasal route of vaccination with VSV-Env also elicited a strong primary response to Env. The primary immune response to Gag elicited by rVSV peaked 7 days after vaccination, at which time 3% of CD8(+) cells were Gag tetramer positive and CD62L(Lo) and functional by intracellular cytokine staining. This response was eightfold higher than that elicited by rVV expressing Gag. VSV-GagEnv, which expresses both Gag and Env from a single recombinant, also induced strong cytotoxic T-lymphocyte (CTL) responses to both Env and Gag. Our quantitative analyses illustrate the potency of the VSV vector system in CTL induction.

MeSH Terms
AIDS Vaccines/administration & dosage,genetics,immunology Animals CD8-Positive T-Lymphocytes/immunology Cytotoxicity, Immunologic Female Gene Products, env/genetics,immunology Gene Products, gag/immunology Genetic Vectors HIV-1/immunology Humans Mice Mice, Inbred BALB C Recombination, Genetic Vaccination Vesicular stomatitis Indiana virus/genetics,immunology
Chemicals
AIDS Vaccines Gene Products, env Gene Products, gag
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Haglund Karl
Department of Pathology, Program in Microbiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Leiner Ingrid
Kerksiek Kristen
Buonocore Linda
Pamer Eric
Rose John K
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.76.6.2730-2738.2002
Published
2002-03-00
Pages
2730-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC136000
Subset
IM
Grants
NIAID NIH HHS · AI24345 · United States
NIAID NIH HHS · AI40357 · United States
NIAID NIH HHS · R37 AI040357 · United States
NIAID NIH HHS · AI42135 · United States
NIAID NIH HHS · R01 AI040357 · United States
NIAID NIH HHS · R01 AI042135 · United States
External Links
PubMed source DOI: 10.1128/jvi.76.6.2730-2738.2002 PMC Full Text View journal details
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