Occurrence and operation of the glycollate--glyoxylate shuttle in mitochondria of Euglena gracilis Z.

The Biochemical journal ·Vol. 184 ·No. 1 ·1979-10-15 ·Pages 189-92

Yokota A, Kitaoka S

Abstract

Both glyoxylate reductase (NADP+) and glycollate dehydrogenase were located exclusively in mitochondria in Euglena gracilis and constitute the glycollate--glyoxylate shuttle, whose existence in higher plants was thought doubtful, owing to different subcellular locations of the two enzymes. Disrupted Euglena mitochondria showed a glycollate-dependent NADPH oxidation, indicating actual operation of the shuttle in this protozoon.

MeSH Terms

Alcohol Oxidoreductases/metabolism Animals Euglena gracilis/enzymology,metabolism Glycolates/metabolism Glyoxylates/metabolism Mitochondria/metabolism

Chemicals

Glycolates Glyoxylates Alcohol Oxidoreductases glyoxylate reductase (NADP+)

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Yokota A

Kitaoka S

References (13)

13 references, click to expand

Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis z.
Biochim Biophys Acta. 1976 Mar 11;429(1):55-62 PMID: 4121
Metabolic regulation of malic enzyme activity from Paracoccus denitrificans by glyoxylate and acetylCoA.
Biochem Biophys Res Commun. 1977 Feb 7;74(3):1116-24 PMID: 14633
Subcellular localization of the GABA-shunt enzymes in Euglena gracilis strain Z.
J Protozool. 1979 Aug;26(3):471-3 PMID: 119850
The chloroplast at work. A review of modern developments in our understanding of chloroplast metabolism.
Prog Biophys Mol Biol. 1978;33(1):1-54 PMID: 415334
Oxidative phosphorylation during glycollate metabolism in mitochondria from phototrophic Euglena gracilis.
Biochem J. 1975 Sep;150(3):373-7 PMID: 813630
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
Purification and properties of two glyoxylate reductases from a species of Pseudomonas.
Biochem J. 1966 Dec;101(3):781-91 PMID: 16742459
A kinetic analysis of coupled enzyme assays.
Biochemistry. 1969 Jul;8(7):2782-6 PMID: 4241273
Localization and properties of hydroxypyruvate and glyoxylate reductases in spinach leaf particles.
J Biol Chem. 1970 Oct 10;245(19):5129-36 PMID: 4394164
Control of the citric acid cycle by glyoxylate. Mechanism of the inhibition by oxalomalate and gamma-hydroxy-alpha-oxoglutarate.
Biochem J. 1967 Apr;103(1):19-23 PMID: 6033757
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.
Biochem J. 1955 Aug;60(4):604-17 PMID: 13249955
Properties of a new glyoxylate reductase from leaves.
Biochem J. 1962 Sep;84:541-6 PMID: 14010085
The localization of glycollate-pathway enzymes in Euglena.
Biochem J. 1975 May;148(2):321-8 PMID: 1156408

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1840189

Published

1979-10-15

Pages

189-92

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1161693

Subset

External Links

PubMed source DOI: 10.1042/bj1840189 PMC Full Text View journal details

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Occurrence and operation of the glycollate--glyoxylate shuttle in mitochondria of Euglena gracilis Z.

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