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PMID: 11875037 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High-density cell microarrays for parallel functional determinations.

Genome research ·Vol. 12 ·No. 3 ·2002-03-00 ·Pages 482-6

Xu CW

Abstract

Whole-genome sequencing projects have generated a wealth of gene sequences from a variety of organisms. A major challenge is to rapidly uncover gene regulatory circuits and their functional manifestations at the cellular level. Here we report the coupled fabrication of nanocraters ranging in size from 100 pL to 1.5 nL on permeable membranes for culturing cells. Using this approach, we developed bacterial and yeast cell microarrays that allowed phenotypic determinations of gene activities and drug targets on a large scale. Cell microarrays will therefore be a particularly useful tool for studying phenotypes of gene activities on a genome-wide scale.

MeSH Terms
Bacteriological Techniques/instrumentation,methods,trends Escherichia coli/cytology,genetics,growth & development Genes, Bacterial/physiology Genes, Fungal/physiology Genome, Bacterial Genome, Fungal Membranes, Artificial Mycology/instrumentation,methods,trends Nanotechnology/instrumentation,methods,trends Saccharomyces cerevisiae/cytology,genetics,growth & development
Chemicals
Membranes, Artificial
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Xu C Wilson
Molecular Pharmacology Program, Memorial Sloan-Kettering Cancer Center and Sloan-Kettering Division, Graduate School of Medical Sciences, Cornell University, New York, NY 10021, USA. [email protected]
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.213002
Email
[email protected]
Published
2002-03-00
Pages
482-6
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC155288
Subset
IM
External Links
PubMed source DOI: 10.1101/gr.213002 PMC Full Text View journal details
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