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PMID: 11877455 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vivo binding of active heat shock transcription factor 1 to human chromosome 9 heterochromatin during stress.

The Journal of cell biology ·Vol. 156 ·No. 5 ·2002-03-04 ·Pages 775-81

Jolly C, Konecny L, Grady DL, Kutskova YA, Cotto JJ, Morimoto RI, Vourc'h C

Abstract

Activation of the mammalian heat shock transcription factor (HSF)1 by stress is a multistep process resulting in the transcription of heat shock genes. Coincident with these events is the rapid and reversible redistribution of HSF1 to discrete nuclear structures termed HSF1 granules, whose function is still unknown. Key features are that the number of granules correlates with cell ploidy, suggesting the existence of a chromosomal target. Here we show that in humans, HSF1 granules localize to the 9q11-q12 heterochromatic region. Within this locus, HSF1 binds through direct DNA-protein interaction with a nucleosome-containing subclass of satellite III repeats. HSF1 granule formation only requires the DNA binding competence and the trimerization of the factor. This is the first example of a transcriptional activator that accumulates transiently and reversibly on a chromosome-specific heterochromatic locus.

MeSH Terms
Binding Sites/genetics Cell Nucleus/genetics,metabolism Cells, Cultured Chromosomes, Human, Pair 9/genetics Cytoplasmic Granules/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Female Fluorescent Antibody Technique Heat Shock Transcription Factors Heat-Shock Proteins/genetics,metabolism Heterochromatin/genetics Humans Microsatellite Repeats/genetics Protein Structure, Tertiary/genetics Stress, Physiological/genetics,metabolism Transcription Factors
Chemicals
DNA-Binding Proteins HSF1 protein, human Heat Shock Transcription Factors Heat-Shock Proteins Heterochromatin Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Jolly Caroline
DyOGen, INSERM U309, Institut A. Bonniot, Domaine de la Merci, 38706 La Tronche cedex, France. [email protected]
Konecny Lara
Grady Deborah L
Kutskova Yulia A
Cotto Jose J
Morimoto Richard I
Vourc'h Claire
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200109018
Email
[email protected]
Published
2002-03-04
Epub
2002-00-04
Pages
775-81
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173303
Subset
IM
Grants
NIGMS NIH HHS · R01 GM038109 · United States
NIGMS NIH HHS · R37 GM038109 · United States
NIGMS NIH HHS · GM38109 · United States
External Links
PubMed source DOI: 10.1083/jcb.200109018 PMC Full Text View journal details
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