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PMID: 1188358 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Guanidinium-CsCl density gradients for isopycnic analysis of nucleic acids.

Science (New York, N.Y.) ·Vol. 190 ·No. 4214 ·1975-11-07 ·Pages 584-6

Enea V, Zinder ND

Abstract

The addition of guanidinium chloride to CsCl gradients lowers the apparent density of RNA, DNA, and hybrid polymers in such a way that all three can be banded, fractionated, and analyzed in one gradient with essentially no damage to their chemical integrity or to their biological activity.

MeSH Terms
Centrifugation, Density Gradient/methods Centrifugation, Isopycnic/methods Cesium Chlorides DNA/analysis Guanidines Nucleic Acid Denaturation Nucleic Acids/analysis RNA/analysis
Chemicals
Chlorides Guanidines Nucleic Acids Cesium RNA DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Enea V
Zinder N D
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.1188358
Published
1975-11-07
Pages
584-6
Language
English
Region
United States
NLM ID
0404511
Subset
IM
External Links
PubMed source DOI: 10.1126/science.1188358 View journal details
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